Department of Medical Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.
Curr Microbiol. 2023 Apr 8;80(5):175. doi: 10.1007/s00284-023-03247-7.
Klebsiella pneumoniae is an opportunistic bacterium, which is globally recognized for its high prevalence and antimicrobial resistance (AMR). Biofilm-forming capability, susceptibility testing, and phenotypic confirmatory test for extended-spectrum beta-lactamase (ESBL)-producing isolate recognition of 104 K. pneumoniae isolates were performed according to the Clinical Laboratory Standard Institute (CLSI) guidelines. The prevalence of ESBL-associated genes bla-VIM, bla-NDM, and bla-OXA-48, as well as biofilm-associated genes luxS, fimH1, wza, and mrkD, was determined by multiplex PCR. The highest resistance rate was against ampicillin (100.0%). Among the 104 K. pneumoniae isolates, 52 (50.0%) and 31 (29.8%) isolates were determined as multi- and extensively drug resistant (MDR, XDR), respectively. Moreover, 21 (40.4%) isolates were determined as ESBL producing. Among 50 biofilm-producing K. pneumoniae isolates, 7 (14.0%), 15 (30.0%), and 28 (56.0%) isolates exhibited high, moderate, and weak levels of biofilm formation, respectively. A number of 41 (78.8%) isolates were susceptible to colistin, and 10 (19.2%) were resistant. AMR was significantly higher (P < 0.05) in the biofilm-forming isolates compared with non-biofilm formers.
肺炎克雷伯菌是一种机会致病菌，其全球流行率高和对抗生素的耐药性（AMR）受到广泛关注。根据临床实验室标准化研究所（CLSI）指南，对 104 株肺炎克雷伯菌进行了生物膜形成能力、药敏试验和表型确证试验，以确认是否为产超广谱β-内酰胺酶（ESBL）的分离株。采用多重 PCR 法检测 bla-VIM、bla-NDM 和 bla-OXA-48 等 ESBL 相关基因以及 luxS、fimH1、wza 和 mrkD 等生物膜相关基因的流行率。104 株肺炎克雷伯菌对氨苄西林的耐药率最高（100.0%）。在 104 株肺炎克雷伯菌中，52 株（50.0%）和 31 株（29.8%）被确定为多药耐药（MDR）和广泛耐药（XDR），此外，21 株（40.4%）被确定为产 ESBL。在 50 株产生物膜的肺炎克雷伯菌中，7 株（14.0%）、15 株（30.0%）和 28 株（56.0%）的生物膜形成能力分别为高、中、低水平。41 株（78.8%）对黏菌素敏感，10 株（19.2%）耐药。与非生物膜形成株相比，生物膜形成株的 AMR 显著更高（P<0.05）。
