Zhong Weihong, Du Xiaolang, Wang Xiaoyun, Cao Lan, Mu Zejing, Zhong Guoyue
Jiangxi University of Chinese Medicine, Nanchang, People's Republic of China.
Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.
Physiol Mol Biol Plants. 2023 Mar;29(3):409-420. doi: 10.1007/s12298-023-01292-x. Epub 2023 Feb 27.
Benth. is an endemic genus in the Himalayas and adjacent areas. Some plants of the genus are traditional medicinal plants in Tibetan medicine. In this study, the chloroplast genomes of five species ( (DC. ex Royle) Good, S. W. Liu, (Hook. f.) Kitam., S. W. Liu, and Diels) were collected for sequencing. The sequencing results showed that the size of the chloroplast genome ranged from 150,985 to 151,284 bp and possessed a typical quadripartite structure containing one large single copy (LSC) region (83,326-83,369 bp), one small single copy (SSC) region (17,956-18,201 bp), and a pair of inverted repeats (IR) regions (24,830-24,855 bp) in , , , , and . The chloroplast genomes encoded an equal number of genes, of which 88 were protein-coding genes, 37 were transfer ribonucleic acid genes, and eight were ribosomal ribonucleic acid genes, and were highly similar in overall size, genome structure, gene content, and order. In comparison with other species in the Asteraceae family, their chloroplast genomes share similarities but show some structural variations. There was no obvious expansion or contraction in the LSC, SSC or IR regions among the five species, indicating that the chloroplast gene structure of the genus was highly conserved. Collinearity analysis showed that there was no gene rearrangement. The results of the phylogenetic tree showed that the whole chloroplast genomes of the five species were closely related, and the plants of this genus were grouped into one large cluster with Cass. and Lindl.
本氏属是喜马拉雅山脉及邻近地区的特有属。该属的一些植物是藏药中的传统药用植物。在本研究中，收集了五个物种（（DC. ex Royle）Good、S. W. Liu、（Hook. f.）Kitam.、S. W. Liu和Diels）的叶绿体基因组进行测序。测序结果表明，叶绿体基因组大小在150,985至151,284 bp之间，具有典型的四分体结构，包含一个大单拷贝（LSC）区域（83,326 - 83,369 bp）、一个小单拷贝（SSC）区域（17,956 - 18,201 bp）以及一对反向重复（IR）区域（24,830 - 24,855 bp），在 、 、 、 和 中。叶绿体基因组编码的基因数量相等，其中88个是蛋白质编码基因，37个是转运核糖核酸基因，8个是核糖体核糖核酸基因，并且在总体大小、基因组结构、基因含量和顺序上高度相似。与菊科的其他物种相比，它们的叶绿体基因组有相似之处，但也存在一些结构变异。五个物种的LSC、SSC或IR区域没有明显的扩张或收缩，表明该属的叶绿体基因结构高度保守。共线性分析表明没有基因重排。系统发育树的结果表明，这五个物种的整个叶绿体基因组密切相关，并且该属的植物与Cass.和Lindl.聚为一个大的类群。
