Medison Milca Banda, Pan Rui, Peng Ying, Medison Rudoviko Galileya, Shalmani Abdullah, Yang XinSun, Zhang Wenying
Research Center of Crop Stresses Resistance Technologies/ Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025 China.
State Key Laboratory of Crop Stress Biology in Arid Areas, College of Life Sciences, Northwest A&F University, Yangling, 712100 China.
Physiol Mol Biol Plants. 2023 Mar;29(3):361-376. doi: 10.1007/s12298-023-01299-4. Epub 2023 Mar 25.
Hydroxycinnamate-CoA quinate hydroxycinnamoyl transferase (HQT) enzyme affect plant secondary metabolism and are crucial for growth and development. To date, limited research on the genome-wide analysis of family genes and their regulatory roles in chlorogenic acid (CGA) accumulation in leafy vegetable sweet potato is available. Here, a total of 58 family genes in the sweet potato genome (named ) were identified and analyzed. We studied the chromosomal distribution, phylogenetic relationship, motifs distribution, collinearity, and cis-acting element analysis of family genes. This study used two sweet potato varieties, high CGA content Fushu 7-6-14-7 (HC), and low CGA content Fushu 7-6 (LC). Based on the phylogenetic analysis, clade A was unique among the identified four clades as it contained genes from various species. The chromosomal location and collinearity analysis revealed that tandem gene duplication may promote the gene expansion and expression. The expression patterns and profile analysis showed changes in gene expression levels at different developmental stages and under cold, drought, and salt stress conditions. The expression analysis verified by qRT-PCR revealed that genes were highly expressed in the HC variety leaves than in the LC variety. Furthermore, cloning and gene function analysis unveiled that family genes are involved in the biosynthesis and accumulation of CGA in sweet-potato. This study expands our understanding of the regulatory role of genes in sweet-potato and lays a foundation for further functional characterization and genetic breeding by engineering targeted candidate genes in various sweet-potato varieties and other species.
The online version contains supplementary material available at 10.1007/s12298-023-01299-4.
羟基肉桂酸 - 辅酶A奎宁酸羟基肉桂酰转移酶（HQT）影响植物次生代谢，对生长发育至关重要。迄今为止，关于叶菜型甘薯中该家族基因的全基因组分析及其在绿原酸（CGA）积累中的调控作用的研究有限。在此，我们在甘薯基因组中鉴定并分析了总共58个该家族基因（命名为 ）。我们研究了该家族基因的染色体分布、系统发育关系、基序分布、共线性和顺式作用元件分析。本研究使用了两个甘薯品种，高CGA含量的福薯7 - 6 - 14 - 7（HC）和低CGA含量的福薯7 - 6（LC）。基于系统发育分析，在已鉴定的四个分支中，A分支是独特的，因为它包含来自不同物种的 基因。染色体定位和共线性分析表明，串联基因重复可能促进 基因的扩增和表达。表达模式和谱分析显示了在不同发育阶段以及冷、旱、盐胁迫条件下基因表达水平的变化。通过qRT - PCR验证的表达分析表明， 基因在HC品种叶片中的表达高于LC品种。此外，克隆和基因功能分析揭示了 家族基因参与甘薯中CGA的生物合成和积累。本研究扩展了我们对甘薯中 基因调控作用的理解，并为通过对各种甘薯品种和其他物种中的目标 候选基因进行工程改造来进一步进行功能表征和遗传育种奠定了基础。
在线版本包含可在10.1007/s12298 - 023 - 01299 - 4获取的补充材料。
