1Department of Pharmacology, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
2Department of Food Hygiene, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran.
Antimicrob Resist Infect Control. 2019 Mar 13;8:54. doi: 10.1186/s13756-019-0505-7. eCollection 2019.
Cockroaches are one of the most important and frequent insects responsible for harboring, transmission and dissemination of human pathogens in the hospital environment. The present research was done to study the phenotypic and genotypic characterization of antibiotic resistance in the Methicillin-resistant strains isolated from hospital cockroaches.
Five-hundred and thirty and cockroaches were collected and their gut content and external washing samples were subjected to bacterial isolation. MRSA strains were subjected to disk diffusion and PCR amplification of antibiotic resistance genes.
Prevalence of MRSA strains in and cockroaches were 52.77 and 43.33%, respectively. External washing samples of cockroaches had the highest prevalence of MRSA strains (59.57%). MRSA isolates of external washing samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), gentamicin (83.33%) and trimethoprim-sulfamethoxazole (80.55%). MRSA strains isolated from gut content samples harbored the highest prevalence of resistance against penicillin (100%), ceftaroline (100%), tetracycline (100%), trimethoprim-sulfamethoxazole (80%) and gentamicin (73.33%). , , , , , , , and were the most commonly detected antibiotic resistance genes amongst the MRSA strains.
The present investigation is the first report of the phenotypic and genotypic evaluation of antibiotic resistance in the MRSA strains isolated from and hospital cockroaches. Hospital cockroaches are considered as a potential mechanical vector for MRSA strains.
蟑螂是在医院环境中携带、传播和传播人类病原体的最重要和最常见的昆虫之一。本研究旨在研究从医院蟑螂中分离的耐甲氧西林金黄色葡萄球菌（MRSA）菌株的表型和基因型耐药特征。
收集了 530 只和 330 只蟑螂，并对其肠道内容物和外部洗涤样本进行细菌分离。MRSA 菌株进行了药敏纸片扩散法和抗生素耐药基因 PCR 扩增检测。
和 蟑螂中 MRSA 菌株的检出率分别为 52.77%和 43.33%。蟑螂外部洗涤样本中 MRSA 菌株的检出率最高（59.57%）。外部洗涤样本的 MRSA 分离株对青霉素（100%）、头孢洛林（100%）、四环素（100%）、庆大霉素（83.33%）和甲氧苄啶-磺胺甲恶唑（80.55%）的耐药率最高。肠道内容物样本中分离的 MRSA 菌株对青霉素（100%）、头孢洛林（100%）、四环素（100%）、甲氧苄啶-磺胺甲恶唑（80%）和庆大霉素（73.33%）的耐药率最高。、、、、、、、和 是 MRSA 菌株中最常见的耐药基因。
本研究首次报道了从和 医院蟑螂中分离的 MRSA 菌株的表型和基因型耐药评估。医院蟑螂被认为是 MRSA 菌株的潜在机械传播媒介。
