Kroupina Aleksandra Yu, Yanovsky Aleksey S, Korobkova Varvara A, Bespalova Ludmila A, Arkhipov Andrey V, Bukreeva Galina I, Voropaeva Anastasiya D, Kroupin Pavel Yu, Litvinov Dmitry Y, Mudrova Aleksandra A, Ulyanov Daniil S, Karlov Gennady I, Divashuk Mikhail G
All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.
P.P. Lukyanenko National Grain Centre, Central Estate of KNIISH, 350012 Krasnodar, Russia.
Foods. 2023 Mar 28;12(7):1436. doi: 10.3390/foods12071436.
Winter durum wheat is a relatively young crop that is highly adaptable due to its winter type of growth habit. The priority of breeding and genetic improvement of winter durum wheat is to improve grain quality and pasta quality, largely determined by the glutenin storage proteins. In the present study, a collection of 76 accessions of winter durum wheat from P.P. Lukyanenko National Grain Centre was studied. The allelic state of high-molecular-weight glutenin genes, and , using PCR markers and SDS-PAGE was identified and grain and pasta quality traits were assessed in a two-year field experiment. The positive effect of the allele and a negative effect of on the gluten index were shown. It was found that and have a positive effect on the quality and quantity of protein and gluten, while the + genotypes were closest to the high-quality category in protein-associated quality traits.
冬性硬粒小麦是一种相对较新的作物，因其冬性生长习性而具有高度适应性。冬性硬粒小麦育种和遗传改良的重点是提高籽粒品质和面食品质，这在很大程度上由谷蛋白贮藏蛋白决定。在本研究中，对来自P.P.卢基扬年科国家谷物中心的76份冬性硬粒小麦种质进行了研究。利用PCR标记和SDS-PAGE鉴定了高分子量谷蛋白基因Glu-A1和Glu-B1的等位基因状态，并在为期两年的田间试验中评估了籽粒和面食品质性状。结果表明，Glu-A1位点的1等位基因对面筋指数有正向作用，Glu-B1位点的7+8等位基因有负向作用。研究发现，Glu-A1位点的1和Glu-B1位点的7对面筋蛋白的品质和数量有正向作用，而Glu-A1位点的1 + Glu-B1位点的7 + 8基因型在蛋白质相关品质性状上最接近优质类别。
