Boldeanu Lucia-Camelia, Popa-Wagner Aurel, Boariu Marius, Stratul Stefan-Ioan, Rusu Darian, Vela Octavia, Roman Alexandra, Surlin Petra, Kardaras Georgios, Chinnici Salvatore, Vaduva Adrian
Department of Periodontology, Faculty of Dental Medicine, Anton Sculean Research Center for Periodontal and Peri-Implant Diseases, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania.
Department of Neurology, Chair of Vascular Neurology and Dementia, University Hospital Essen, 45147 Essen, Germany.
J Clin Med. 2023 Mar 23;12(7):2462. doi: 10.3390/jcm12072462.
Tissue breakdown was assessed by confocal laser scanning microscopy (CLSM) using autofluorescence around implants with ligatures, on a dog hemimandible. Influence of section thickness on the accuracy of histometrical observations was also evaluated, in comparison with thin sections in light microscopy.
Three months after tooth extraction, implants were placed. Two months after abutment placement, ligatures were placed with no plaque control. 11 months post-implantation, the animal was sacrificed. Undecalcified thin (30 µm) sections were cut, stained and evaluated by light microscopy to be used as a reference. Additional sections were performed, so that another pair of unstained thick sections resulted (250-300 µm). Tissue loss was assessed using histomorphometric parameters under CLSM and was compared to the light microscopy reference ones.
Morphometry confirmed tissue loss more pronounced on the "thick" and quick sections, when compared to the time-consuming and technique-sensitive "thin" ones.
Within the limits of the present study, the adequacy of histometrical observations under CLSM reveal commensurable information about soft-tissue-bone-implant details, when compared to traditional light microscopy histological protocols. The CLSM investigation may seem demanding, yet the richness of data acquired may justify this approach, provided seatbacks caused by improper manipulation of "thick" sections are avoided.
使用共聚焦激光扫描显微镜（CLSM），通过观察犬半侧下颌骨上带有结扎线的种植体周围的自发荧光，评估组织分解情况。还与光学显微镜下的薄片进行比较，评估切片厚度对组织计量学观察准确性的影响。
拔牙3个月后植入种植体。基台放置2个月后，在不进行菌斑控制的情况下放置结扎线。植入后11个月，处死动物。切取未脱钙的薄片（30μm），进行染色并通过光学显微镜评估，用作参考。制作额外的切片，从而得到另一对未染色的厚切片（250 - 300μm）。使用CLSM下的组织形态计量学参数评估组织损失，并与光学显微镜参考参数进行比较。
形态计量学证实，与耗时且技术敏感的“薄”切片相比，“厚”切片和快速切片上的组织损失更明显。
在本研究的范围内，与传统光学显微镜组织学方法相比，CLSM下组织计量学观察的充分性揭示了有关软组织 - 骨 - 种植体细节的可比信息。CLSM研究可能看起来要求较高，但只要避免因“厚”切片操作不当导致的问题，所获取数据的丰富性可能证明这种方法是合理的。
