A 14-day randomized controlled study with a parallel design was conducted with 80 healthy participants. Intervention groups I (IG1) and II (IG2) received a defined background diet and consumed a smoothie enriched with either 15 g of dry weight (d.w.) or 15 g of d.w. daily. Control group II (CG2) received a defined background diet without the smoothie. Control group I (CG1) received neither. Blood samples and 24-h urine were collected at the beginning and the end of the study. Serum concentrations of 25-hydroxyvitamin D, vitamin D, selenium, iron, ferritin, transferrin saturation, total cholesterol, low-density lipoprotein (LDL) cholesterol, high-density lipoprotein (HDL) cholesterol, non-HDL cholesterol and the LDL-cholesterol/HDL cholesterol ratio decreased in IG1 ( < 0.05), while 25-hydroxyvitamin D increased ( < 0.05). In IG2, vitamin D, 25-hydroxyvitamins D and D decreased ( < 0.05), while concentrations of fatty acids C20:5 and C22:5 increased. Serum and urine uric acid increased in IG1 and IG2 ( < 0.05). is a valuable source of n3 fatty acids, as is of vitamin D. Regular consumption of may affect the iron and selenium status negatively but may impact blood lipids positively. An elevated uric acid concentration in blood and urine following the regular consumption of microalgae poses potential risks for human health.