Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka 72388, Saudi Arabia.
Department of Surgery, College of Medicine, Majmaah University, Al-Majmaah 11952, Saudi Arabia.
Molecules. 2023 Apr 4;28(7):3228. doi: 10.3390/molecules28073228.
Breast cancer is among the most recurrent malignancies, and its prevalence is rising. With only a few treatment options available, there is an immediate need to search for better alternatives. In this regard, nanotechnology has been applied to develop potential chemotherapeutic techniques, particularly for cancer therapy. Specifically, albumin-based nanoparticles are a developing platform for the administration of diverse chemotherapy drugs owing to their biocompatibility and non-toxicity. Visnagin, a naturally derived furanochromone, treats cancers, epilepsy, angina, coughs, and inflammatory illnesses. In the current study, the synthesis and characterization of albumin visnagin (AV) nanoparticles (NPs) using a variety of techniques such as transmission electron microscopy, UV-visible, Fourier transform infrared, energy dispersive X-ray composition analysis, field emission scanning electron microscopy, photoluminescence, X-Ray diffraction, and dynamic light scattering analyses have been carried out. The MTT test, dual AO/EB, DCFH-DA, Annexin-V-FITC/PI, Propidium iodide staining techniques as well as analysis of apoptotic proteins, antioxidant enzymes, and PI3K/Akt/mTOR signaling analysis was performed to examine the NPs' efficacy to suppress MDA-MB-468 cell lines. The NPs decreased cell viability increased the amount of ROS in the cells, disrupted membrane integrity, decreased the level of antioxidant enzymes, induced cell cycle arrest, and activated the PI3K/Akt/mTOR signaling cascade, ultimately leading to cell death. Thus, AV NPs possesses huge potential to be employed as a strong anticancer therapy alternative.
乳腺癌是最常见的恶性肿瘤之一，其发病率正在上升。由于仅有少数治疗选择可用，因此迫切需要寻找更好的替代方法。在这方面，纳米技术已被应用于开发潜在的化疗技术，特别是用于癌症治疗。具体来说，基于白蛋白的纳米粒子因其生物相容性和低毒性而成为多种化疗药物给药的新兴平台。醉鱼草素是一种天然衍生的呋喃色酮，可治疗癌症、癫痫、心绞痛、咳嗽和炎症性疾病。在当前的研究中，使用多种技术（如透射电子显微镜、紫外可见分光光度法、傅里叶变换红外光谱、能量色散 X 射线成分分析、场发射扫描电子显微镜、光致发光、X 射线衍射和动态光散射分析）对白蛋白醉鱼草素（AV）纳米粒子（NPs）进行了合成和表征。通过 MTT 试验、双 AO/EB、DCFH-DA、Annexin-V-FITC/PI、碘化丙啶染色技术以及凋亡蛋白、抗氧化酶和 PI3K/Akt/mTOR 信号分析，研究了 NPs 抑制 MDA-MB-468 细胞系的功效。NPs 降低了细胞活力，增加了细胞内 ROS 的含量，破坏了膜的完整性，降低了抗氧化酶的水平，诱导细胞周期停滞，并激活了 PI3K/Akt/mTOR 信号级联，最终导致细胞死亡。因此，AV NPs 具有作为强大的抗癌治疗替代物的巨大潜力。
