The First Clinical College, Chongqing Medical University, Chongqing, China.
Department of Nursing, University Town Hospital Affiliated to Chongqing Medical University, Chongqing, China.
PLoS One. 2023 Apr 13;18(4):e0274277. doi: 10.1371/journal.pone.0274277. eCollection 2023.
Coronary artery disease (CAD) is a leading cause of death worldwide. Many studies in China and abroad have reported an association between the expression level of microRNA-155 and CAD; however, the results remain controversial. We aimed to comprehensively investigate this association based on a meta-analysis.
We first systematically searched eight Chinese and English databases, including China National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, PubMed, Web of Science, Embase, Google Scholar, and Cochrane Library, to identify studies concerning the relationship between microRNA-155 levels and CAD published before February 7, 2021. The quality of the literature was assessed by the Newcastle-Ottawa Scale (NOS). Meta-analysis was performed using a random-effects model to calculate the standard mean difference with a 95% confidence interval (CI).
Sixteen articles with a total of 2069 patients with CAD and 1338 controls were included. All the articles were of high quality according to the NOS. The meta-analysis showed that the mean level of microRNA-155 was significantly lower in patients with CAD than in controls. Based on subgroup analyses, the level of microRNA-155 in the plasma of CAD patients and in acute myocardial infarction (AMI) patients was significantly lower than that in controls, whereas this level in CAD patients with mild stenosis was significantly higher than that in controls.
Our study indicates that the expression level of circulating microRNA-155 in patients with CAD is lower than that in a non-CAD group, suggesting a new possible reference index for the diagnosis and monitoring of patients with CAD.
冠心病（CAD）是全球范围内主要的死亡原因。国内外许多研究报道了 microRNA-155 的表达水平与 CAD 之间存在关联，但结果仍存在争议。我们旨在通过荟萃分析全面研究这种关联。
我们首先系统地检索了 8 个中英文数据库，包括中国知识基础设施、万方、中国科技期刊数据库、PubMed、Web of Science、Embase、Google Scholar 和 Cochrane Library，以确定截至 2021 年 2 月 7 日发表的关于 microRNA-155 水平与 CAD 之间关系的研究。文献质量采用 Newcastle-Ottawa 量表（NOS）进行评估。使用随机效应模型进行荟萃分析，计算标准均数差和 95%置信区间（CI）。
纳入了 16 篇文章，共 2069 例 CAD 患者和 1338 例对照。根据 NOS，所有文章的质量均较高。荟萃分析显示，CAD 患者的 microRNA-155 平均水平明显低于对照组。基于亚组分析，CAD 患者和急性心肌梗死（AMI）患者的血浆 microRNA-155 水平明显低于对照组，而轻度狭窄的 CAD 患者的水平明显高于对照组。
我们的研究表明，CAD 患者循环 microRNA-155 的表达水平低于非 CAD 组，提示其可能成为 CAD 患者诊断和监测的新的潜在参考指标。
