College of Basic Medical Sciences, Hubei University of Chinese Medicine, Huangjiahu West Road 16, Wuhan 430065, PR China.
Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, PR China; Hubei Province Academy of Traditional Chinese Medicine, Wuhan 430074, PR China.
Int J Biol Macromol. 2023 Jun 30;241:124386. doi: 10.1016/j.ijbiomac.2023.124386. Epub 2023 Apr 11.
In this study, two homogeneous polysaccharides (APS-A1 and APS-B1) were isolated from Astragalus membranaceus by DEAE-52 cellulose and Sephadex G-100 column chromatography. Their chemical structures were characterized by molecular weight distribution, monosaccharide composition, infrared spectrum, methylation analysis, and NMR. The results revealed that APS-A1 (2.62 × 10 Da) was a 1,4-α-D-Glcp backbone with a 1,4,6-α-D-Glcp branch every ten residues. APS-B1 (4.95 × 10 Da) was a heteropolysaccharide composed of glucose, galactose, and arabinose (75.24:17.27:19.35). Its backbone consisted of 1,4-α-D-Glcp, 1,4,6-α-D-Glcp, 1,5-α-L-Araf and the sidechains composed of 1,6-α-D-Galp and T-α/β-Glcp. Bioactivity assays showed that APS-A1 and APS-B1 had potential anti-inflammatory activity. They could inhibit the production of inflammatory factors (TNF-α, IL-6, and MCP-1) in LPS-stimulated RAW264.7 macrophages via NF-κB and MAPK (ERK, JNK) pathways. These results suggested that the two polysaccharides could be potential anti-inflammatory supplements.
在这项研究中，两种均一多糖（APS-A1 和 APS-B1）通过 DEAE-52 纤维素和 Sephadex G-100 柱色谱从黄芪中分离得到。它们的化学结构通过分子量分布、单糖组成、红外光谱、甲基化分析和 NMR 进行了表征。结果表明，APS-A1（2.62×10 Da）是具有 1,4-α-D-Glcp 主链的多糖，每隔十个残基就有一个 1,4,6-α-D-Glcp 支链。APS-B1（4.95×10 Da）是一种由葡萄糖、半乳糖和阿拉伯糖（75.24:17.27:19.35）组成的杂多糖。其主链由 1,4-α-D-Glcp、1,4,6-α-D-Glcp、1,5-α-L-Araf 组成，侧链由 1,6-α-D-Galp 和 T-α/β-Glcp 组成。活性测定表明，APS-A1 和 APS-B1 具有潜在的抗炎活性。它们可以通过 NF-κB 和 MAPK（ERK、JNK）途径抑制 LPS 刺激的 RAW264.7 巨噬细胞中炎症因子（TNF-α、IL-6 和 MCP-1）的产生。这些结果表明，这两种多糖可能是潜在的抗炎补充剂。
