Dani A M, Cittadini A, Calviello G, Festuccia G, Terranova T
Mol Cell Biochem. 1978 Dec 22;22(2-3):139-45. doi: 10.1007/BF00496239.
Isolated rat thymocytes incubated under proper metabolic conditions extrude Ca2+ previously taken up under metabolically unfavourable conditions. The extrusion can be supported by both respiratory and glycolytic energy but glycolysis seems to be more efficient for this purpose. La3+ (50--200 micron) and the ionophore A 23187 inhibit cell Ca2+ extrusion. Ruthenium Red (1--100 micron) does not influence cell Ca2+ extrusion while it inhibits the in situ mitochondrial cation uptake. All the results are consistent with a cell regulation model of Ca2+ content in which both plasma membrane and mitochondria co-operate, acting in opposite directions, in order to decrease cytosolic Ca2+ concentration. The possibility of Na+-Ca2+ hetero-exchange participation to cell Ca2+ homeostasis regulation is also discussed.
在适当代谢条件下孵育的离体大鼠胸腺细胞会排出先前在代谢不利条件下摄取的Ca2+。呼吸能量和糖酵解能量都能支持这种排出，但糖酵解在此方面似乎更有效。La3+（50 - 200微米）和离子载体A 23187会抑制细胞Ca2+的排出。钌红（1 - 100微米）不影响细胞Ca2+的排出，但会抑制原位线粒体阳离子的摄取。所有结果都与一种Ca2+含量的细胞调节模型一致，在该模型中，质膜和线粒体相互协作，以相反方向作用，从而降低细胞质Ca2+浓度。文中还讨论了Na+-Ca2+异向交换参与细胞Ca2+稳态调节的可能性。
