一种用于多色光纤光度测定和光遗传学操作的灵活多用系统。
A flexible and versatile system for multi-color fiber photometry and optogenetic manipulation.
机构信息
Research Group Synaptic Wiring and Information Processing, Center for Molecular Neurobiology Hamburg, University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany.
Department of Neurophysiology, MCTN, Medical Faculty Mannheim, Heidelberg University, 68167 Mannheim, Germany.
出版信息
Cell Rep Methods. 2023 Mar 7;3(3):100418. doi: 10.1016/j.crmeth.2023.100418. eCollection 2023 Mar 27.
Here, we present simultaneous fiber photometry recordings and optogenetic stimulation based on a multimode fused fiber coupler for both light delivery and collection without the need for dichroic beam splitters. In combination with a multi-color light source and appropriate optical filters, our approach offers remarkable flexibility in experimental design and facilitates the exploration of new molecular tools at minimal cost. We demonstrate straightforward re-configuration of the setup to operate with green, red, and near-infrared calcium indicators with or without simultaneous optogenetic stimulation and further explore the multi-color photometry capabilities of the system. The ease of assembly, operation, characterization, and customization of this platform holds the potential to foster the development of experimental strategies for multi-color fused fiber photometry combined with optogenetics far beyond its current state.
在这里，我们展示了一种基于多模光纤耦合器的同时光纤光度记录和光遗传学刺激方法，无需使用二向色分束镜即可实现光的传输和收集。结合多色光源和适当的光学滤波器，我们的方法在实验设计方面具有显著的灵活性，并以最低的成本促进了新分子工具的探索。我们演示了如何通过简单的重新配置来实现绿色、红色和近红外钙指示剂的操作，同时还可以进行或不进行同时的光遗传学刺激，并进一步探索系统的多色光度测量能力。该平台易于组装、操作、特性描述和定制，有望促进多色光纤光度测量与光遗传学相结合的实验策略的发展，使其超越当前的状态。
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