Joseph Sandeep J, Bommana Sankhya, Ziklo Noa, Kama Mike, Dean Deborah, Read Timothy D
Division of STD Prevention, Centers for Disease Control and Prevention, Atlanta, GA, United States.
Department of Pediatrics, University of California, San Francisco, Oakland, CA, United States.
Front Microbiol. 2023 Mar 28;14:1154664. doi: 10.3389/fmicb.2023.1154664. eCollection 2023.
, a gram-negative obligate intracellular bacterium, commonly causes sexually transmitted infections (STIs). Little is known about transmission within the host, which is important for understanding disease epidemiology and progression.
We used RNA-bait enrichment and whole-genome sequencing to compare rectal, vaginal and endocervical samples collected at the same time from 26 study participants who attended Fijian Ministry of Health and Medical Services clinics and tested positive for at each anatomic site.
The 78 genomes from participants resolved into two major clades of the phylogeny (the "prevalent urogenital and anorectal" clade and "non-prevalent urogenital and anorectal" clade). For 21 participants, genome sequences were almost identical in each anatomic site. For the other five participants, two distinct strains were present in different sites; in two cases, the vaginal sample was a mixture of strains.
The absence of large numbers of fixed SNPs between genomes within many of the participants could indicate recent acquisition of infection prior to the clinic visit without sufficient time to accumulate significant genetic variation in different body sites. This model suggests that many infections may be resolved relatively quickly in the Fijian population, possibly reflecting common prescription or over-the-counter antibiotics usage.
[病原体名称]是一种革兰氏阴性专性胞内细菌，通常引起性传播感染（STIs）。关于其在宿主体内的传播情况知之甚少，而这对于理解疾病流行病学和进展至关重要。
我们使用RNA诱饵富集和全基因组测序，对来自26名研究参与者的直肠、阴道和宫颈样本进行比较，这些参与者前往斐济卫生和医疗服务部诊所就诊，且在每个解剖部位检测出[病原体名称]呈阳性。
参与者的78个[病原体名称]基因组解析为该病原体系统发育的两个主要分支（“常见泌尿生殖道和肛门直肠”分支以及“非常见泌尿生殖道和肛门直肠”分支）。对于21名参与者，每个解剖部位的基因组序列几乎相同。对于其他五名参与者，不同部位存在两种不同的[病原体名称]菌株；在两例中，阴道样本为菌株混合物。
许多参与者体内的[病原体名称]基因组之间缺乏大量固定单核苷酸多态性（SNPs），这可能表明在就诊前近期感染，且没有足够时间在不同身体部位积累显著的遗传变异。该模型表明，在斐济人群中，许多[病原体名称]感染可能相对较快得到解决，这可能反映了常见的处方或非处方抗生素使用情况。
