Opt Lett. 2023 Apr 15;48(8):2042-2045. doi: 10.1364/OL.482403.
We report the development of a novel line-scanning microscope capable of acquiring high-speed time-correlated single-photon counting (TCSPC)-based fluorescence lifetime imaging microscopy (FLIM) imaging. The system consists of a laser-line focus, which is optically conjugated to a 1024 × 8 single-photon avalanche diode (SPAD)-based line-imaging complementary metal-oxide semiconductor (CMOS), with 23.78 µm pixel pitch at 49.31% fill factor. Incorporation of on-chip histogramming on the line-sensor enables acquisition rates 33 times faster than our previously reported bespoke high-speed FLIM platforms. We demonstrate the imaging capability of the high-speed FLIM platform in a number of biological applications.
我们开发了一种新型线扫描显微镜，能够进行高速基于时间相关单光子计数（TCSPC）的荧光寿命成像显微镜（FLIM）成像。该系统由激光线焦点组成，与基于 1024×8 单光子雪崩二极管（SPAD）的线成像互补金属氧化物半导体（CMOS）光学共轭，在 49.31%填充因子下具有 23.78μm 的像素间距。在线传感器上集成的片上直方图功能使采集速度比我们之前报道的定制高速 FLIM 平台快 33 倍。我们在多个生物学应用中展示了高速 FLIM 平台的成像能力。
