College of Material and Chemical Engineering, Minjiang University, Fuzhou 350108, China; College of Environment and Safety Engineering, Fuzhou university, Fuzhou 350108, China.
College of Material and Chemical Engineering, Minjiang University, Fuzhou 350108, China; Fujian Engineering Research Center of New Chinese Lacquer Materials, Fuzhou 350108, China.
Carbohydr Polym. 2023 Jul 15;312:120792. doi: 10.1016/j.carbpol.2023.120792. Epub 2023 Mar 9.
Due to the large specific surface area and rich pore structure, chitosan nanofiber membrane has many advantages over conventional gel-like or film-like products. However, the poor stability in acidic solutions and relatively weak antibacterial activity against Gram-negative bacteria severely restrict its use in many industries. Here, we present a chitosan-urushiol composite nanofiber membrane prepared by electrospinning. Chemical and morphology characterization revealed that the formation of chitosan-urushiol composite involved the Schiff base reaction between catechol and amine groups and the self-polymerization of urushiol. The unique crosslinked structure and multiple antibacterial mechanisms endowed the chitosan-urushiol membrane with outstanding acid resistance and antibacterial performance. After immersion in HCl solution at pH 1, the membrane maintained its intact appearance and satisfactory mechanical strength. In addition to its good antibacterial performance against Gram-positive Staphylococcus aureus (S. aureus), the chitosan-urushiol membrane exhibited synergistic antibacterial activity against Gram-negative Escherichia coli (E. coli) that far exceeded that of neat chitosan membrane and urushiol. Moreover, cytotoxicity and hemolysis assays revealed that the composite membrane had good biocompatibility similar to that of neat chitosan. In short, this work provides a convenient, safe, and environmentally friendly method to simultaneously enhance the acid resistance and broad-spectrum antibacterial activity of chitosan nanofiber membranes.
由于具有较大的比表面积和丰富的孔结构，壳聚糖纳米纤维膜比传统的凝胶状或膜状产品具有许多优势。然而，其在酸性溶液中稳定性差，对革兰氏阴性菌的抗菌活性相对较弱，严重限制了其在许多行业中的应用。在这里，我们通过静电纺丝制备了一种壳聚糖-漆酚复合纳米纤维膜。化学和形貌表征表明，壳聚糖-漆酚复合的形成涉及邻苯二酚和胺基之间的席夫碱反应以及漆酚的自聚合。独特的交联结构和多种抗菌机制赋予了壳聚糖-漆酚膜出色的耐酸性和抗菌性能。在 pH 1 的 HCl 溶液中浸泡后，膜保持完整的外观和令人满意的机械强度。除了对革兰氏阳性金黄色葡萄球菌（S. aureus）具有良好的抗菌性能外，壳聚糖-漆酚膜对革兰氏阴性大肠杆菌（E. coli）表现出协同抗菌活性，远远超过了纯壳聚糖膜和漆酚。此外，细胞毒性和溶血试验表明，复合膜具有与纯壳聚糖相似的良好生物相容性。总之，这项工作提供了一种方便、安全、环保的方法，可以同时提高壳聚糖纳米纤维膜的耐酸性和广谱抗菌活性。
