Shinagawa Akiho, Yamazaki Tomoki, Minematsu Ayako, Serizawa Naho, Hosoi Yuri, Ninomiya Yusuke, Miyakoshi Yuichi, Yano Tomohiro, Ota Masako
Graduate School of Food and Nutritional Sciences, Toyo University, 1-1-1, Izumino, Itakura-Cho, Oragun, Gunma, 374-0193, Japan.
Institute of Life Innovation Studies, Toyo University, Oragun, Gunma, 374-0193, Japan.
BMC Sports Sci Med Rehabil. 2023 Apr 17;15(1):59. doi: 10.1186/s13102-023-00656-w.
Acute exercise is one factor that increases blood homocysteine levels, and elevated homocysteine levels cause oxidative stress. Albumin, which is abundant in blood, is an antioxidant, and the redox state of albumin is used as an index of oxidative stress in blood. This study aimed to assess the effect of acute exercise on plasma homocysteine levels and the blood non-mercaptoalbumin/mercaptoalbumin ratio as an oxidative stress marker.
This study used a crossover design with exercise and control conditions. Under exercise conditions, a bicycle ergometer was used to perform 40 min of transient constant-load exercise at 65% heart rate reserve. Under control conditions, participants rested for 40 min. Blood was collected before, 30 min after, and 90 min after exercise, and at the same time points under control conditions. Samples were analyzed for the homocysteine concentration and non-mercaptoalbumin/mercaptoalbumin ratio.
The results revealed that a 65% heart rate reserve and 40 min of acute exercise increased plasma homocysteine concentration and non-mercaptoalbumin ratio. In the intra-condition comparison, the plasma Hcy concentration was significantly increased at Post 30 min (+ 0.83 ± 0.70 µmol/L, P = 0.003) compared with that at Pre in the exercise condition. Furthermore, 90 min after exercise, the blood non-mercaptoalbumin ratio was significantly increased (+ 0.35 ± 0.71%, P = 0.030) compared to Pre.
These results indicate that the plasma Hcy concentration first increased, and then the non-mercaptoalbumin/mercaptoalbumin ratio increased as the elevated state was maintained. This study revealed that 65% heart rate reserve, 40 min of acute exercise increased plasma Hcy concentration and non-mercaptoalbumin ratio.
急性运动是导致血液中同型半胱氨酸水平升高的一个因素，而同型半胱氨酸水平升高会引起氧化应激。血液中丰富的白蛋白是一种抗氧化剂，白蛋白的氧化还原状态被用作血液中氧化应激的指标。本研究旨在评估急性运动对血浆同型半胱氨酸水平和作为氧化应激标志物的血液非巯基白蛋白/巯基白蛋白比值的影响。
本研究采用运动和对照条件的交叉设计。在运动条件下，使用自行车测力计以心率储备的65%进行40分钟的短暂恒负荷运动。在对照条件下，参与者休息40分钟。在运动前、运动后30分钟和90分钟以及对照条件下的相同时间点采集血液。对样本进行同型半胱氨酸浓度和非巯基白蛋白/巯基白蛋白比值分析。
结果显示，心率储备的65%以及40分钟的急性运动可使血浆同型半胱氨酸浓度和非巯基白蛋白比值升高。在组内比较中，与运动前相比，运动后30分钟血浆同型半胱氨酸浓度显著升高（+0.83±0.70µmol/L，P = 0.003）。此外，运动后90分钟，血液非巯基白蛋白比值与运动前相比显著升高（+0.35±0.71%，P = 0.030）。
这些结果表明，血浆同型半胱氨酸浓度首先升高，然后随着升高状态的维持，非巯基白蛋白/巯基白蛋白比值升高。本研究表明，心率储备的65%、40分钟的急性运动可使血浆同型半胱氨酸浓度和非巯基白蛋白比值升高。
