Cell Electrophysiology Laboratory, Wannan Medical College, Wuhu, 241002, Anhui, China.
Stroke Center and Department of Neurology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230036, Anhui, China.
J Nanobiotechnology. 2022 Aug 1;20(1):348. doi: 10.1186/s12951-022-01550-2.
Fullerenol, a functional and water-soluble fullerene derivative, plays an important role in antioxidant, antitumor and antivirus, implying its enormous potential in biomedical applications. However, the in vivo performance of fullerenol remains largely unclear. We aimed to investigate the effect of fullerenol (i.p., 5 mg/kg) on the impaired hippocampus in a rat model of lead exposure. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a kind of newly developed soft-ionization mass spectrometry technology. In the present study, an innovative strategy for biological distribution analysis using MALDI-TOF-MS confirmed that fullerenol could across the blood-brain barrier and accumulate in the brain. Results from behavioral tests showed that a low dose of fullerenol could improve the impaired learning and memory induced by lead. Furthermore, electrophysiology examinations indicated that this potential repair effect of fullerenol was mainly due to the long-term changes in hippocampal synaptic plasticity, with enhancement lasting for more than 2-3 h. In addition, morphological observations and biochemistry analyses manifested that the long-term change in synaptic efficacy was accompanied by some structural alteration in synaptic connection. Our study demonstrates the therapeutic feature of fullerenol will be beneficial to the discovery and development as a new drug and lays a solid foundation for further biomedical applications of nanomedicines.
富勒醇是一种功能性的、水溶性的富勒烯衍生物，在抗氧化、抗肿瘤和抗病毒方面发挥着重要作用，这暗示了其在生物医学应用中具有巨大的潜力。然而，富勒醇的体内性能在很大程度上仍不清楚。我们旨在研究富勒醇（腹腔注射，5mg/kg）对暴露于铅的大鼠模型中海马损伤的影响。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF-MS）是一种新开发的软电离质谱技术。在本研究中，使用 MALDI-TOF-MS 的生物分布分析的创新策略证实，富勒醇可以穿透血脑屏障并在大脑中积累。行为测试的结果表明，低剂量的富勒醇可以改善铅引起的学习和记忆损伤。此外，电生理学检查表明，富勒醇的这种潜在修复作用主要归因于海马突触可塑性的长期变化，其增强作用持续超过 2-3 小时。此外，形态学观察和生物化学分析表明，突触效能的长期变化伴随着突触连接的一些结构改变。我们的研究表明，富勒醇的治疗特征将有益于新药的发现和开发，并为纳米药物的进一步生物医学应用奠定了坚实的基础。
