Rahman Mohammad Shamsur, Currò Sarah, Carraro Lisa, Cardazzo Barbara, Balzan Stefania, Novelli Enrico, Fontana Federico, Caburlotto Greta, Manfrin Amedeo, Fasolato Luca
Aquatic Animal Health Group, Department of Fisheries, Faculty of Biological Sciences, University of Dhaka, Dhaka, Bangladesh.
Department of Comparative Biomedicine and Food Science, University of Padua, Legnaro, Italy.
Ital J Food Saf. 2023 Mar 8;12(1):11045. doi: 10.4081/ijfs.2023.11045.
The genus includes bacteria with different morphological and metabolic characteristics responsible for different human and animal diseases. An accurate identification is essential to assess the risks in regard to aquatic organisms and consequently to public health. The Multilocus Sequence Analysis (MLSA) scheme developed on the basis of 4 housekeeping genes (B, H, A and A) was applied to identify 92 strains isolated from crustaceans in 2011. Concatenated sequences were used for the phylogenetic and population analyses and the results were compared with those from biochemical identification tests. From the phylogenetic analysis, 10 clusters and 4 singletons emerged, whereas the population analysis highlighted 12 subpopulations that were well supported by phylogeny with few exceptions. The retrospective analysis allowed correct re-attribution of isolated species, indicating how, for some pathogens, there may be an overestimation of phenotypic identification (. ). Use of the PubMLST database highlighted a possible genetic link between Sequence Type (ST) 529 and ST195 () isolated from a human case in Norway during 2018. In addition to the identification of major risk groups of , and , MLSA could be a valid support for species considered a minor risk, such as , and . Due to the increased incidence of vibriosis in Europe, the application of different tools will also have to be considered to investigate the possible epidemiological links of the various species in the perspective of to protect the consumer.
该属包括具有不同形态和代谢特征的细菌，这些细菌可引发不同的人类和动物疾病。准确鉴定对于评估水生生物的风险以及进而评估对公众健康的风险至关重要。基于4个管家基因（B、H、A和A）开发的多位点序列分析（MLSA）方案被应用于鉴定2011年从甲壳类动物中分离出的92株菌株。串联序列用于系统发育和群体分析，并将结果与生化鉴定试验的结果进行比较。从系统发育分析中，出现了10个簇和4个单株，而群体分析突出了12个亚群体，除少数例外，这些亚群体在系统发育上得到了很好的支持。回顾性分析允许对分离出的物种进行正确的重新归类，表明对于某些病原体，可能存在表型鉴定的高估（......）。使用PubMLST数据库突出了2018年在挪威从一例人类病例中分离出的序列类型（ST）529和ST195（......）之间可能存在的遗传联系。除了鉴定主要的风险组......和......之外，MLSA对于被认为是低风险的物种，如......、......和......，可能是一种有效的支持。由于欧洲弧菌病发病率的增加，还必须考虑应用不同的工具，从......的角度调查各种物种可能的流行病学联系，以保护消费者。
