LncRNA regulates axis to promote the progression of non-small cell lung cancer.

BACKGROUND

Non-small cell lung cancer (NSCLC) is the most common malignancy in lung cancer, with a low survival rate and unfavorable prognosis. Dysregulated long non-coding RNAs (lncRNAs) play vital functions in tumor progression. This study intended to probe the expression pattern and function of in NSCLC.

METHODS

Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted to analyze the expression of , , , mRNA-decapping enzyme 1A (), and . Cell viability, migration, and invasion were separately examined via 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) and transwell experiments. Luciferase reporter assay was conducted to evaluate the binding of with or . Protein expression of was assessed via Western blot. NSCLC animal models were constructed through injection of H1975 cells transfected with lentivirus (LV)-sh-HOXD-AS2 into nude mice, followed by hematoxylin and eosin (HE) staining and immunohistochemistry (IHC) analysis.

RESULTS

In this study, was upregulated in NSCLC tissues and cells, and high predicted short overall survival (OS). Downregulation of could impair the proliferation, migration, and invasion abilities of H1975 and A549 cells. was shown to bind with and be lowly expressed in NSCLC. Suppression of could abolish the inhibitory effect of silencing on proliferation, migration, and invasion. was screened as the target of and its overexpression could rescue upregulation-repressed proliferation, migration, and invasion activities. Moreover, animal experiments affirmed that promoted tumor growth .

CONCLUSIONS

modulates the / axis to boost the progression of NSCLC, which founds the basis of as a new diagnostic biomarker and molecular target for NSCLC therapy.