Adarkwa Eben, Roy Abhijit, Ohodnicki John, Lee Boeun, Kumta Prashant N, Desai Salil
Center of Excellence in Product Design and Advanced Manufacturing, North Carolina Agricultural and Technical State University, Greensboro, North Carolina, USA.
Department of Bioengineering, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Int J Bioprint. 2023 Jan 4;9(2):661. doi: 10.18063/ijb.v9i2.661. eCollection 2023.
Three-dimensional (3D) printing is implemented for surface modification of titanium alloy substrates with multilayered biofunctional polymeric coatings. Poly(lactic-co-glycolic) acid (PLGA) and polycaprolactone (PCL) polymers were embedded with amorphous calcium phosphate (ACP) and vancomycin (VA) therapeutic agents to promote osseointegration and antibacterial activity, respectively. PCL coatings revealed a uniform deposition pattern of the ACP-laden formulation and enhanced cell adhesion on the titanium alloy substrates as compared to the PLGA coatings. Scanning electron microscopy and Fourier-transform infrared spectroscopy confirmed a nanocomposite structure of ACP particles showing strong binding with the polymers. Cell viability data showed comparable MC3T3 osteoblast proliferation on polymeric coatings as equivalent to positive controls. live/dead assessment indicated higher cell attachments for 10 layers (burst release of ACP) as compared to 20 layers (steady release) for PCL coatings. The PCL coatings loaded with the antibacterial drug VA displayed a tunable release kinetics profile based on the multilayered design and drug content of the coatings. Moreover, the concentration of active VA released from the coatings was above the minimum inhibitory concentration and minimum bactericidal concentration, demonstrating its effectiveness against bacterial strain. This research provides a basis for developing antibacterial biocompatible coatings to promote osseointegration of orthopedic implants.
采用三维（3D）打印技术对钛合金基底进行表面改性，制备多层生物功能聚合物涂层。聚乳酸-乙醇酸共聚物（PLGA）和聚己内酯（PCL）聚合物分别与无定形磷酸钙（ACP）和万古霉素（VA）治疗剂复合，以分别促进骨整合和抗菌活性。与PLGA涂层相比，PCL涂层显示出负载ACP配方的均匀沉积模式，并增强了在钛合金基底上的细胞粘附。扫描电子显微镜和傅里叶变换红外光谱证实了ACP颗粒与聚合物形成了强结合的纳米复合结构。细胞活力数据显示，聚合物涂层上MC3T3成骨细胞的增殖与阳性对照相当。活/死评估表明，PCL涂层10层（ACP突发释放）的细胞附着量高于20层（稳定释放）。负载抗菌药物VA的PCL涂层根据涂层的多层设计和药物含量显示出可调的释放动力学曲线。此外，从涂层释放的活性VA浓度高于最低抑菌浓度和最低杀菌浓度，证明了其对细菌菌株的有效性。本研究为开发促进骨科植入物骨整合的抗菌生物相容性涂层提供了依据。
