Rozman Nur Amiera Syuhada, Yenn Tong Woei, Ring Leong Chean, Ab Rashid Syarifah, Wen-Nee Tan, Lim Jun Wei
Universiti Kuala Lumpur, Branch Campus Malaysian Institute of Chemical and Bioengineering Technology, Lot 1988 Kawasan Perindustrian Bandar Vendor, Taboh Naning, 78000 Alor Gajah, Melaka, Malaysia.
Universiti Kuala Lumpur - Institute of Medical Science Technology, A1, 1, Jalan TKS 1, Taman Kajang Sentral, 43000 Kajang, Selangor.
Trop Life Sci Res. 2023 Mar;34(1):279-291. doi: 10.21315/tlsr2023.34.1.15. Epub 2023 Mar 31.
Microbial infection is a frequent complication of diabetic foot ulcers, with up to 82% of ulcers being infected at the initial stage of diabetes. Furthermore, the emergence of beta lactam resistant pathogens managed to eliminate the use of beta lactam antibiotics as a chemotherapeutic alternative. This further increases the amputation and mortality rate. Hence, the aim of this study is to evaluate antimicrobial efficacy of a ketone derivative 2-octylcyclopentanone against diabetic wound pathogens. The inhibitory activity of the compound was determined using disc diffusion and broth microdilution assay. Generally, 2-octylcyclopentanone showed broad-spectrum antimicrobial activity, particularly against beta lactam resistant pathogens. The compound showed comparably better antimicrobial activity than all reference antibiotics, including chloramphenicol, streptomycin, ampicillin and penicillin. In addition, the same compound also inhibits a clinically isolated that was resistant to all reference antibiotics. The activity was microbicidal based on the low minimal lethality concentration recorded, particularly on MRSA, and . The killing efficiency of the compound was concentration dependent. During kill curve analysis, the inhibitory activity of 2-octylcyclopentanone was concentration and time-dependent. 99.9% of reduction of bacterial growth was observed. MRSA and , two significant diabetic wound infections, are totally inhibited by the molecule at a concentration of minimum lethality concentration. In short, 2-octylcyclopentanone exhibited significant inhibitory towards wide range of diabetic wound pathogens. Which is considered crucial since it will provide a safe and effective alternative treatment for diabetic ulcer infection.
微生物感染是糖尿病足溃疡常见的并发症，在糖尿病初期，高达82%的溃疡会受到感染。此外，β-内酰胺耐药病原体的出现使得β-内酰胺类抗生素作为化疗药物的选择不再可行。这进一步增加了截肢率和死亡率。因此，本研究旨在评估酮衍生物2-辛基环戊酮对糖尿病伤口病原体的抗菌效果。使用纸片扩散法和肉汤微量稀释法测定该化合物的抑制活性。总体而言，2-辛基环戊酮具有广谱抗菌活性，尤其对β-内酰胺耐药病原体。该化合物显示出比所有参考抗生素（包括氯霉素、链霉素、氨苄西林和青霉素）更好的抗菌活性。此外，该化合物还能抑制一株对所有参考抗生素均耐药的临床分离菌。基于所记录的低最低致死浓度，该活性具有杀菌作用，特别是对耐甲氧西林金黄色葡萄球菌（MRSA）以及[此处原文缺失一种细菌名称]。该化合物的杀菌效率呈浓度依赖性。在杀菌曲线分析中，2-辛基环戊酮的抑制活性呈浓度和时间依赖性。观察到细菌生长减少了99.9%。MRSA以及[此处原文缺失一种细菌名称]这两种严重的糖尿病伤口感染，在最低致死浓度下被该分子完全抑制。简而言之，2-辛基环戊酮对多种糖尿病伤口病原体表现出显著的抑制作用。鉴于它将为糖尿病溃疡感染提供一种安全有效的替代治疗方法，这一点至关重要。
