Jo Se Jin, Gong Yue, Jiang Rui, Jung Hyunjae, Kim Young Ran
College of Pharmacy and Research Institute of Drug Development, Chonnam National University, Gwangju 61186, Korea.
Jaimdang Bio, Gyeonggi 10945, Korea.
Prev Nutr Food Sci. 2023 Mar 31;28(1):21-29. doi: 10.3746/pnf.2023.28.1.21.
The prevalence of obesity has been increasing worldwide, and its pathogenesis is closely related to preadipo-cyte differentiation. Because the presence of obesity increases the risk of chronic disease, it is important to decrease exces-sive body fat accumulation. This study aimed to demonstrate the anti-adipogenesis and anti-obesity effects of gongmi tea and gongmi so extract. The 3T3-L1 preadipocyte cell line was stained with Oil red O, and the expression levels of peroxi-some proliferator-activated receptor-γ (PPARγ), adiponectin, and fatty acid-binding protein 4 (FABP4) were evaluated via Western blot analysis. A mouse model of obesity was developed by feeding C57BL/6 male mice a high-fat diet (HFD). Gongmi tea or gongmi so extract was orally administered at a dose of 200 mg/kg for 6 weeks. The mouse body weight was measured weekly during the study period, and the epididymal adipose tissue weight and blood serum were analyzed at the end of the study period. The gongmi tea and gongmi so extract did not exhibit toxicity in mice. Oil red O staining showed that gongmi tea significantly decreased excessive body fat accumulation. In addition, gongmi tea (300 μg/mL) significantly downregulated adipogenic transcription factors, such as PPARγ, adiponectin, and FABP4. tests indicated that oral administration of gongmi tea or gongmi so extract to C57BL/6 mice with HFD-induced obesity effectively decreased their body weight and epididymal adipose tissue. Gongmi tea and gongmi so extract have potent anti-adipogenic effects in 3T3-L1 cells and anti-obesity effects in mice with HFD-induced obesity.
肥胖症在全球范围内的患病率一直在上升，其发病机制与前脂肪细胞分化密切相关。由于肥胖的存在会增加患慢性病的风险，减少过多的体脂积累非常重要。本研究旨在证明贡米茶叶和贡米籽提取物的抗脂肪生成和抗肥胖作用。用 Oil red O 对 3T3-L1 前脂肪细胞系进行染色，并通过蛋白质免疫印迹分析评估过氧化物酶体增殖物激活受体γ（PPARγ）、脂联素和脂肪酸结合蛋白 4（FABP4）的表达水平。通过给 C57BL/6 雄性小鼠喂食高脂饮食（HFD）建立肥胖小鼠模型。以 200 mg/kg 的剂量口服给予贡米茶叶或贡米籽提取物，持续 6 周。在研究期间每周测量小鼠体重，并在研究期结束时分析附睾脂肪组织重量和血清。贡米茶叶和贡米籽提取物在小鼠中未表现出毒性。Oil red O 染色显示，贡米茶叶显著减少了过多的体脂积累。此外，贡米茶叶（300 μg/mL）显著下调了 PPARγ、脂联素和 FABP4 等脂肪生成转录因子。试验表明，给高脂饮食诱导肥胖的 C57BL/6 小鼠口服贡米茶叶或贡米籽提取物可有效降低其体重和附睾脂肪组织重量。贡米茶叶和贡米籽提取物在 3T3-L1 细胞中具有强大的抗脂肪生成作用，在高脂饮食诱导肥胖的小鼠中具有抗肥胖作用。
