Wu P H, Phillis J W
Neurochem Res. 1978 Oct;3(5):563-71. doi: 10.1007/BF00963759.
The steady-state level of brain ATP was measured after the tissue had been treated with a focused microwave irradiation system. The brain ATP content (1.50 nmol/mg tissue) obtained by microwave fixation is similar to that observed by others using fast-freezing and microwave fixation techniques. The concentrations of ATP in different brain regions show a rather uniform distribution, ranging from 1.918 +/- 0.059 (brainstem) to 2.393 +/- 0.19 (caudate) nmol/mg tissue; however, insufficient microwave fixation time seems to produce a greater regional variation of ATP. Release of ATP was investigated by placing a cup on the sensory-motor cortex. The rate for basal release of ATP is 1.43 +/- 0.14 femtomole/min/mm(2). A 30-fold increase in ATP release was obtained by direct stimulation of the cortex with 5 mA pulses of 0.2 msec duration at a rate of 20/sec over a period of 10 min. These results, in conjunction with others describing the potent pharmacological action of the nucleotide, seem to suggest that ATP could be a mediator in a "purinergic" system in the CNS.
在用聚焦微波照射系统处理组织后，测量了脑ATP的稳态水平。通过微波固定获得的脑ATP含量（1.50 nmol/mg组织）与其他人使用快速冷冻和微波固定技术观察到的结果相似。不同脑区的ATP浓度呈现出相当均匀的分布，范围从1.918±0.059（脑干）到2.393±0.19（尾状核）nmol/mg组织；然而，微波固定时间不足似乎会导致ATP的区域差异更大。通过在感觉运动皮层放置一个杯子来研究ATP的释放。ATP基础释放速率为1.43±0.14飞摩尔/分钟/平方毫米。通过以20次/秒的频率、0.2毫秒持续时间的5 mA脉冲直接刺激皮层10分钟，ATP释放增加了30倍。这些结果与其他描述核苷酸强大药理作用的结果相结合，似乎表明ATP可能是中枢神经系统中“嘌呤能”系统的一种介质。
