McCandless D W, Stavinoha W B, Abel M S
Brain Res Bull. 1984 Aug;13(2):253-5. doi: 10.1016/0361-9230(84)90125-4.
A method is described in which brain tissue from microwave heat inactivated mouse brain is prepared for microregional energy metabolite analysis. This method permits the sectioning of the tissue into sections 40 microns thick in which layers can be visualized and dissected. Results show no movement or leaching of metabolites from one microregion to another. This method therefore, permits the combined use of the two powerful neurochemical techniques of microwave irradiation for sacrifice and microregional analysis for energy metabolite determination.
本文描述了一种方法，该方法用于制备来自经微波热灭活的小鼠大脑的脑组织，以进行微区能量代谢物分析。这种方法可以将组织切成40微米厚的切片，在这些切片中可以看到并解剖各层。结果表明代谢物没有从一个微区移动或渗漏到另一个微区。因此，这种方法允许联合使用两种强大的神经化学技术，即微波辐照用于处死动物和微区分析用于确定能量代谢物。
