Department of Medicine, School of Clinical Medicine, The University of Hong Kong, Hong Kong, China.
School of Biomedical Science, The University of Hong Kong, Hong Kong, China.
Nat Commun. 2023 Apr 17;14(1):2196. doi: 10.1038/s41467-023-37975-y.
Transient gut microbiota alterations have been reported after antibiotic therapy for Helicobacter pylori. However, alteration in the gut virome after H. pylori eradication remains uncertain. Here, we apply metagenomic sequencing to fecal samples of 44 H. pylori-infected patients at baseline, 6-week (N = 44), and 6-month (N = 33) after treatment. Following H. pylori eradication, we discover contraction of the gut virome diversity, separation of virome community with increased community difference, and shifting towards a higher proportion of core virus. While the gut microbiota is altered at 6-week and restored at 6-month, the virome community shows contraction till 6-month after the treatment with enhanced phage-bacteria interactions at 6-week. Multiple courses of antibiotic treatments further lead to lower virus community diversity when compared with treatment naive patients. Our results demonstrate that H. pylori eradication therapies not only result in transient alteration in gut microbiota but also significantly alter the previously less known gut virome community.
抗生素治疗幽门螺杆菌后，肠道微生物组会发生短暂改变。然而，幽门螺杆菌根除后肠道病毒组的改变仍不确定。在这里，我们应用宏基因组测序技术对 44 名幽门螺杆菌感染患者的粪便样本进行分析，分别在基线时、治疗 6 周后（N=44）和 6 个月后（N=33）进行。幽门螺杆菌根除后，我们发现肠道病毒组的多样性收缩，病毒组群落分离，群落差异增加，核心病毒的比例上升。虽然肠道微生物组在 6 周时发生改变，并在 6 个月时恢复，但病毒组群落直到治疗后 6 个月仍在收缩，且在 6 周时噬菌体-细菌相互作用增强。与未接受治疗的患者相比，多次接受抗生素治疗后，病毒群落的多样性降低。我们的研究结果表明，幽门螺杆菌根除疗法不仅会导致肠道微生物组的短暂改变，还会显著改变先前较少被关注的肠道病毒组群落。
