人原核延伸复合物的结构。
Structures of human primosome elongation complexes.
机构信息
Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.
Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
出版信息
Nat Struct Mol Biol. 2023 May;30(5):579-583. doi: 10.1038/s41594-023-00971-3. Epub 2023 Apr 17.
Abstract
The synthesis of RNA-DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA-DNA primers.
摘要
原核起始前复合物（primosome）通过引发酶（primase）和 DNA 聚合酶 α 亚基的协调作用合成 RNA-DNA 引物，在此过程中伴随着多个结构域的未知构象重排。本研究使用低温电子显微镜解析了一个 3.6 Å 的人类原核起始前复合物结构，该结构处于脱氧核苷酸延伸的早期阶段。该结构证实了引发酶大亚基的长期作用，并揭示了原核起始前复合物如何限制合成短 RNA-DNA 引物的新见解。
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