College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Institute of Biomedical Sciences, Shandong Normal University, Jinan, 250014, P. R. China.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China.
Angew Chem Int Ed Engl. 2023 Jun 19;62(25):e202302161. doi: 10.1002/anie.202302161. Epub 2023 May 9.
Monitoring the early stage of atherosclerosis (AS) without plaque formation is of great significance. Herein, we developed a metal organic framework (MOF)-based fluorescence nanoprobe to analyze the progression of AS by assessing the levels of protein phosphorylation and glucose in blood and tissue. The probe was prepared by post-modification of the MOF with iodine (I )-rhodamine B (RhB) associate, which realizes the specific recognition of target object through the metal joint Zr and I -RhB, respectively. We investigated different stages of target object changes in the early non-plaque stage of AS in blood. It was found that the levels of phosphate and glucose in the blood were higher than those of the normal mice. The results of two-photon images showed that early AS mice had higher levels of protein phosphorylation and glucose than that of the normal mice. The present study provides a suitable fluorescence tool for further revealing the pathogenesis and progression of AS.
监测无斑块形成的动脉粥样硬化（AS）早期阶段具有重要意义。在此，我们开发了一种基于金属有机骨架（MOF）的荧光纳米探针，通过分析血液和组织中蛋白磷酸化和葡萄糖的水平来分析 AS 的进展。该探针是通过用碘（I ）-罗丹明 B（RhB）配合物对 MOF 进行后修饰制备的，分别通过金属键合 Zr 和 I -RhB 实现对目标物的特异性识别。我们研究了不同阶段的目标物在 AS 的早期非斑块阶段在血液中的变化。结果发现，血液中磷酸盐和葡萄糖的水平高于正常小鼠。双光子图像的结果表明，早期 AS 小鼠的蛋白磷酸化和葡萄糖水平高于正常小鼠。本研究为进一步揭示 AS 的发病机制和进展提供了一种合适的荧光工具。
