• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

HhaI甲基化对DNA局部结构的影响。

The effect of HhaI methylation on DNA local structure.

作者信息

Fox K R

出版信息

Biochem J. 1986 Feb 15;234(1):213-6. doi: 10.1042/bj2340213.

DOI:10.1042/bj2340213
PMID:3707542
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146547/
Abstract

The effect of methylating the central cytosine in the sequence GCGC on the sensitivity to digestion by deoxyribonuclease I was investigated. Examination of five such sites shows that the extra methyl group renders the bond on its 5' side more susceptible to cleavage, and this is interpreted by suggesting that it causes small changes in the local DNA phosphate orientation.

摘要

研究了在序列GCGC中对中央胞嘧啶进行甲基化修饰对脱氧核糖核酸酶I消化敏感性的影响。对五个这样的位点进行检测发现，额外的甲基使该位点5'端的磷酸二酯键更易被切割，对此的解释是，甲基化导致了局部DNA磷酸基团取向发生微小变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3586/1146547/157a07b13d87/biochemj00285-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3586/1146547/c3ee56c5c5ec/biochemj00285-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3586/1146547/157a07b13d87/biochemj00285-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3586/1146547/c3ee56c5c5ec/biochemj00285-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3586/1146547/157a07b13d87/biochemj00285-0210-a.jpg

相似文献

1
The effect of HhaI methylation on DNA local structure.HhaI甲基化对DNA局部结构的影响。
Biochem J. 1986 Feb 15;234(1):213-6. doi: 10.1042/bj2340213.
2
HhaI methylase and restriction endonuclease as probes for B to Z DNA conformational changes in d(GCGC) sequences.以HhaI甲基化酶和限制性内切酶作为检测d(GCGC)序列中B型至Z型DNA构象变化的探针。
Nucleic Acids Res. 1984 Oct 25;12(20):7677-92. doi: 10.1093/nar/12.20.7677.
3
Alterations in DNA helix stability due to base modifications can be evaluated using denaturing gradient gel electrophoresis.由于碱基修饰导致的DNA螺旋稳定性改变可通过变性梯度凝胶电泳进行评估。
J Mol Biol. 1987 Dec 20;198(4):737-44. doi: 10.1016/0022-2836(87)90214-2.
4
A 7-Deazaadenosylaziridine Cofactor for Sequence-Specific Labeling of DNA by the DNA Cytosine-C5 Methyltransferase M.HhaI.一种用于DNA胞嘧啶-C5甲基转移酶M.HhaI对DNA进行序列特异性标记的7-脱氮腺苷基氮丙啶辅因子。
Molecules. 2015 Nov 23;20(11):20805-22. doi: 10.3390/molecules201119723.
5
Nucleosides and nucleotides. CXIX. Inhibition of DNA-cytosine methylase HhaI by a self-complementary oligonucleotide containing 5-fluorocytosine.
Biol Pharm Bull. 1993 Jun;16(6):529-33. doi: 10.1248/bpb.16.529.
6
Mechanism of inhibition of DNA (cytosine C5)-methyltransferases by oligodeoxyribonucleotides containing 5,6-dihydro-5-azacytosine.含5,6-二氢-5-氮杂胞嘧啶的寡脱氧核糖核苷酸对DNA（胞嘧啶C5）-甲基转移酶的抑制机制
J Mol Biol. 1999 Feb 5;285(5):2021-34. doi: 10.1006/jmbi.1998.2426.
7
Inhibition of HhaI DNA (Cytosine-C5) methyltransferase by oligodeoxyribonucleotides containing 5-aza-2'-deoxycytidine: examination of the intertwined roles of co-factor, target, transition state structure and enzyme conformation.含5-氮杂-2'-脱氧胞苷的寡脱氧核糖核苷酸对HhaI DNA（胞嘧啶-C5）甲基转移酶的抑制作用：对辅因子、靶点、过渡态结构和酶构象相互交织作用的研究
J Mol Biol. 2002 Oct 11;323(1):53-67. doi: 10.1016/s0022-2836(02)00918-x.
8
Impact of benzo[a]pyrene-2'-deoxyguanosine lesions on methylation of DNA by SssI and HhaI DNA methyltransferases.苯并[a]芘-2'-脱氧鸟苷损伤对SssI和HhaI DNA甲基转移酶介导的DNA甲基化的影响。
Biochemistry. 2006 May 16;45(19):6142-59. doi: 10.1021/bi0511639.
9
S-adenosyl-L-methionine-dependent methyl transfer: observable precatalytic intermediates during DNA cytosine methylation.S-腺苷-L-甲硫氨酸依赖性甲基转移：DNA胞嘧啶甲基化过程中可观察到的催化前中间体。
Biochemistry. 2007 Jul 31;46(30):8766-75. doi: 10.1021/bi7005948. Epub 2007 Jul 7.
10
Dynamic impact of methylation at the M. Hhai target site: a solid-state deuterium NMR study.M. Hhai靶位点甲基化的动态影响：一项固态氘核磁共振研究。
Biochemistry. 2001 Oct 16;40(41):12436-43. doi: 10.1021/bi0102555.

引用本文的文献

1
Base Dynamics in the I Protein Binding Site.I 蛋白结合位点的基础动力学。
J Phys Chem B. 2023 Aug 24;127(33):7266-7275. doi: 10.1021/acs.jpcb.3c03687. Epub 2023 Aug 10.
2
Probing DNA shape and methylation state on a genomic scale with DNase I.利用 DNA 酶 I 在基因组范围内探测 DNA 形状和甲基化状态。
Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6376-81. doi: 10.1073/pnas.1216822110. Epub 2013 Apr 1.
3
Nuclear extracts of chicken embryos promote an active demethylation of DNA by excision repair of 5-methyldeoxycytidine.

本文引用的文献

1
Reversible bending and helix geometry in a B-DNA dodecamer: CGCGAATTBrCGCG.B-DNA十二聚体中的可逆弯曲和螺旋几何结构：CGCGAATTBrCGCG
J Biol Chem. 1982 Dec 25;257(24):14686-707.
2
DNA structural variations produced by actinomycin and distamycin as revealed by DNAase I footprinting.通过DNA酶I足迹法揭示的放线菌素和偏端霉素产生的DNA结构变异。
Nucleic Acids Res. 1984 Dec 21;12(24):9271-85. doi: 10.1093/nar/12.24.9271.
3
Sequence preferences in the binding to DNA of triostin A and TANDEM as reported by DNase I footprinting.如通过DNA酶I足迹法所报告的，曲古抑菌素A和串联体与DNA结合时的序列偏好性。
鸡胚的核提取物通过5-甲基脱氧胞苷的切除修复促进DNA的主动去甲基化。
Proc Natl Acad Sci U S A. 1993 May 15;90(10):4684-8. doi: 10.1073/pnas.90.10.4684.
4
Differences in the accessibility of methylated and unmethylated DNA to DNase I.甲基化和未甲基化DNA对DNase I的可及性差异。
Nucleic Acids Res. 1993 Dec 25;21(25):5843-5. doi: 10.1093/nar/21.25.5843.
5
Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro.单纯疱疹病毒胸苷激酶启动子CTF和Sp1识别位点的胞嘧啶甲基化：对体内转录和体外因子结合的影响
Nucleic Acids Res. 1989 Dec 25;17(24):10179-90. doi: 10.1093/nar/17.24.10179.
6
Binding of the transcription factor EBP-80 mediates the methylation response of an intracisternal A-particle long terminal repeat promoter.转录因子EBP - 80的结合介导了脑内A颗粒长末端重复启动子的甲基化反应。
Mol Cell Biol. 1991 Jan;11(1):117-25. doi: 10.1128/mcb.11.1.117-125.1991.
FEBS Lett. 1984 Oct 29;176(2):414-20. doi: 10.1016/0014-5793(84)81209-0.
4
DNA methylation patterns. Formation and function.DNA甲基化模式。形成与功能。
Biochim Biophys Acta. 1984 Sep 10;782(4):331-42. doi: 10.1016/0167-4781(84)90043-5.
5
DNA structural variations in the E. coli tyrT promoter.大肠杆菌tyrT启动子中的DNA结构变异
Cell. 1984 Jun;37(2):491-502. doi: 10.1016/0092-8674(84)90379-9.
6
DNA methylation and gene activity.DNA甲基化与基因活性。
Annu Rev Biochem. 1983;52:93-124. doi: 10.1146/annurev.bi.52.070183.000521.
7
Pattern of methylation of two genes coding for housekeeping functions.两个编码管家功能的基因的甲基化模式。
Proc Natl Acad Sci U S A. 1983 May;80(9):2422-6. doi: 10.1073/pnas.80.9.2422.
8
Structure of a B-DNA dodecamer. II. Influence of base sequence on helix structure.B型DNA十二聚体的结构。II. 碱基序列对螺旋结构的影响。
J Mol Biol. 1981 Jul 15;149(4):761-86. doi: 10.1016/0022-2836(81)90357-0.
9
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).甲基化对合成多核苷酸的影响：聚（dG-m5dC）·聚（dG-m5dC）中的B-Z转变
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1619-23. doi: 10.1073/pnas.78.3.1619.
10
DNA methylation and its possible biological roles.DNA甲基化及其可能的生物学作用。
Prog Nucleic Acid Res Mol Biol. 1981;25:33-52. doi: 10.1016/s0079-6603(08)60482-1.