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甲基化和未甲基化DNA对DNase I的可及性差异。

Differences in the accessibility of methylated and unmethylated DNA to DNase I.

作者信息

Kochanek S, Renz D, Doerfler W

机构信息

Institute of Genetics, University of Cologne, Germany.

出版信息

Nucleic Acids Res. 1993 Dec 25;21(25):5843-5. doi: 10.1093/nar/21.25.5843.

DOI:10.1093/nar/21.25.5843
PMID:8290342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC310463/
Abstract

DNase I binds in the minor groove of DNA and is used as an enzymatic tool to investigate the interaction of proteins with DNA. Here we show that the major groove located 5-methyldeoxycytidine can enhance or inhibit the cleavage rates of DNA by DNase I. This effect may be caused in part by changes in DNA structure affecting the accessibility of the minor groove of DNA to DNase I.

摘要

脱氧核糖核酸酶I（DNase I）结合于DNA的小沟，用作研究蛋白质与DNA相互作用的酶学工具。我们在此表明，位于5-甲基脱氧胞苷处的大沟可增强或抑制DNase I对DNA的切割速率。这种效应可能部分是由于DNA结构的变化影响了DNA小沟对DNase I的可及性所致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0419/310463/b6e002b8e409/nar00074-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0419/310463/d1e9d7d67b9b/nar00074-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0419/310463/b6e002b8e409/nar00074-0049-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0419/310463/d1e9d7d67b9b/nar00074-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0419/310463/b6e002b8e409/nar00074-0049-b.jpg

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本文引用的文献

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Probing DNA-protein interactions in vitro with the CpG DNA methyltransferase.利用CpG DNA甲基转移酶体外探测DNA-蛋白质相互作用
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DNA methylation in the Alu sequences of diploid and haploid primary human cells.二倍体和单倍体原代人类细胞Alu序列中的DNA甲基化
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Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6376-81. doi: 10.1073/pnas.1216822110. Epub 2013 Apr 1.
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The topology of the promoter of RNA polymerase II- and III-transcribed genes is modified by the methylation of 5'-CG-3' dinucleotides.RNA聚合酶II和III转录基因启动子的拓扑结构通过5'-CG-3'二核苷酸的甲基化而发生改变。
Nucleic Acids Res. 1994 Jul 11;22(13):2568-75. doi: 10.1093/nar/22.13.2568.
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5
DNA methylation and gene activity.DNA甲基化与基因活性。
Annu Rev Biochem. 1983;52:93-124. doi: 10.1146/annurev.bi.52.070183.000521.
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