Department of Biology & Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, College of Science, Shantou University, Shantou, Guangdong 515063, PR China.
Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea; Institute for High Technology Materials and Devices, Korea University, Seoul 02841, Republic of Korea.
Int J Biol Macromol. 2023 Jun 30;241:124490. doi: 10.1016/j.ijbiomac.2023.124490. Epub 2023 Apr 17.
Polysaccharides κ-carrageenan (κ-Car) have become a predominant source in developing bioactive materials. We aimed to develop biopolymer composite materials of κ-Car with coriander essential oil (CEO) (κ-Car-CEO) films for fibroblast-associated wound healing. Initially, we loaded the CEO in to κ-Car and CEO through homogenization and ultrasonication to fabricate composite film bioactive materials. After performing morphological and chemical characterizations, we validated the developed material functionalities in both in vitro and in vivo models. The chemical and morphological analysis with physical structure, swelling ratio, encapsulation efficiency, CEO release, and water barrier properties of films examined and showed the structural interaction of κ-Car and CEO-loaded into the polymer network. Furthermore, the bioactive applications of CEO release showed initial burst release followed by controlled release from the κ-Car composite film with fibroblast (L929) cell adhesive capabilities and mechanosensing. Our results proved that the CEO-loaded into the κ-Car film impacts cell adhesion, F-actin organization, and collagen synthesis, followed by in vitro mechanosensing activation, further promoting wound healing in vivo. Our innovative perspectives of active polysaccharide (κ-Car)-based CEO functional film materials could potentially accomplish regenerative medicine.
角叉菜胶（κ-Car）多糖已成为开发生物活性材料的主要来源。我们旨在开发具有芫荽精油（CEO）的κ-Car 生物聚合物复合膜（κ-Car-CEO），用于成纤维细胞相关的伤口愈合。最初，我们通过均化和超声处理将 CEO 加载到 κ-Car 和 CEO 中，以制备复合膜生物活性材料。在进行形态和化学特性分析后，我们在体外和体内模型中验证了所开发材料的功能。通过对薄膜的物理结构、溶胀比、包封效率、CEO 释放和水屏障性能进行化学和形态分析，表明了κ-Car 和负载到聚合物网络中的 CEO 的结构相互作用。此外，CEO 释放的生物活性应用显示出初始突释，随后从具有成纤维细胞（L929）细胞黏附能力和机械传感的 κ-Car 复合膜中进行控制释放。我们的结果证明，负载到 κ-Car 薄膜中的 CEO 会影响细胞黏附、F-肌动蛋白组织和胶原蛋白合成，随后在体外机械传感激活，进一步促进体内伤口愈合。我们对角叉菜胶（κ-Car）为基础的 CEO 功能薄膜材料的创新观点，可能会实现再生医学。
