Medical Functional Experiment Center, North Sichuan Medical College, Nanchong, 637007, People's Republic of China.
Department of Pharmacy, Affiliated Hospital of North Sichuan Medical College, Nanchong, 637000, People's Republic of China.
Sci Rep. 2023 Apr 19;13(1):6383. doi: 10.1038/s41598-023-33228-6.
Lysozyme can kill bacteria by its enzymatic activity or through a mechanism involving its cationic nature, which can facilitate electrostatic interactions with the viral capsid, the negatively charged parts of nucleic acids, and polymerase, so binding to nucleic acids may be another biological function of lysozyme. Here, PCR was used as a research tool to detect the effects of lysozyme on the replication and transcription of nucleic acids after treatment in different ways. We found that lysozyme and its hydrolysate can enter cells and inhibit PCR to varying degrees in vitro, and degraded lysozyme inhibited nucleic acid replication more effectively than intact lysozyme. The inhibition of lysozyme may be related to polymerase binding, and the sensitivity of different polymerases to lysozyme is inconsistent. Our findings provide a theoretical basis for further explaining the pharmacological effects of lysozyme, such as antibacterial, antiviral, anticancer, and immune regulatory activities, and directions for the development of new pharmacological effects of lysozyme and its metabolites.
溶菌酶可以通过其酶活性或通过涉及其阳离子性质的机制来杀死细菌，这可以促进与病毒衣壳、核酸的带负电荷部分和聚合酶的静电相互作用，因此与核酸的结合可能是溶菌酶的另一种生物学功能。在这里，PCR 被用作研究工具，以检测溶菌酶在不同处理方式下对核酸复制和转录的影响。我们发现溶菌酶及其水解产物可以进入细胞，并在体外以不同程度抑制 PCR，并且降解的溶菌酶比完整的溶菌酶更有效地抑制核酸复制。溶菌酶的抑制可能与聚合酶结合有关，不同聚合酶对溶菌酶的敏感性不一致。我们的发现为进一步解释溶菌酶的药理作用，如抗菌、抗病毒、抗癌和免疫调节活性提供了理论依据，并为开发溶菌酶及其代谢物的新药理作用指明了方向。
