Floess Moritz, Steinle Tobias, Werner Florian, Wang Yunshan, Wagner Willi L, Steinle Verena, Liu Betty S, Zheng Yifan, Chen Zi, Ackermann Maximilian, Mentzer Steven J, Giessen Harald
4 Physics Institute and Stuttgart Research Center of Photonic Engineering, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany.
Department of Diagnostic and Interventional Radiology, University Hospital of Heidelberg, Im Neuenheimer Feld 420, 69120 Heidelberg, Germany.
Biomed Opt Express. 2023 Mar 7;14(4):1460-1471. doi: 10.1364/BOE.485314. eCollection 2023 Apr 1.
Pectin is a heteropolysaccharide responsible for the structural integrity of the cell walls of terrestrial plants. When applied to the surface of mammalian visceral organs, pectin films form a strong physical bond with the surface glycocalyx. A potential mechanism of pectin adhesion to the glycocalyx is the water-dependent entanglement of pectin polysaccharide chains with the glycocalyx. A better understanding of such fundamental mechanisms regarding the water transport dynamics in pectin hydrogels is of importance for medical applications, e.g., surgical wound sealing. We report on the water transport dynamics in hydrating glass-phase pectin films with particular emphasis on the water content at the pectin-glycocalyceal interface. We used label-free 3D stimulated Raman scattering (SRS) spectral imaging to provide insights into the pectin-tissue adhesive interface without the confounding effects of sample fixation, dehydration, shrinkage, or staining.
果胶是一种杂多糖，负责陆地植物细胞壁的结构完整性。当应用于哺乳动物内脏器官表面时，果胶膜与表面糖萼形成牢固的物理结合。果胶与糖萼粘附的一种潜在机制是果胶多糖链与糖萼的水依赖性缠结。更好地理解果胶水凝胶中水传输动力学的此类基本机制对于医学应用（例如手术伤口封闭）很重要。我们报告了水合玻璃相果胶膜中的水传输动力学，特别强调了果胶 - 糖萼界面处的含水量。我们使用无标记三维受激拉曼散射（SRS）光谱成像来深入了解果胶 - 组织粘附界面，而不受样品固定、脱水、收缩或染色的混杂影响。
