由特定寡脱氧核糖核苷酸序列引发的抗体。

Antibodies elicited by defined oligodeoxyribonucleotide sequences.

作者信息

Störl H J, Simon H, Barthelmes H

出版信息

Nucleic Acids Res. 1978 Dec;5(12):4919-31. doi: 10.1093/nar/5.12.4919.

DOI:10.1093/nar/5.12.4919

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342798/

Abstract

Antibodies to the oligodeoxyribonucleotides d(pT)3, d(pT)4, d(pT)6 and d(pA-A-T-T) were elicited in rabbits by immunization with electrostatic complexes of the respective haptens with methylated bovine serum albumin (MBSA). The antisera were assayed by complement fixation using denatured DNA's of various sources as antigens. The specificities of the antibodies were determined by estimating the inhibition of the complement fixation reaction by defined oligodeoxyribonucleotides. The antibodies were shown to be specific for the sequence of the oligode-oxyribonucleotides or parts of it.

摘要

通过将各自的半抗原与甲基化牛血清白蛋白（MBSA）形成的静电复合物免疫家兔，可诱导产生针对寡脱氧核糖核苷酸d(pT)3、d(pT)4、d(pT)6和d(pA - A - T - T)的抗体。使用各种来源的变性DNA作为抗原，通过补体结合试验检测抗血清。通过测定特定寡脱氧核糖核苷酸对补体结合反应的抑制作用来确定抗体的特异性。结果表明，这些抗体对寡脱氧核糖核苷酸的序列或其部分序列具有特异性。

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