针对两种脱氧核糖三核苷酸序列的特异性抗体。
Antibodies specific to two deoxyribotrinucleotide sequences.
作者信息
Khan S A, Jacob T M
出版信息
Nucleic Acids Res. 1977 Sep;4(9):3007-15. doi: 10.1093/nar/4.9.3007.
Abstract
Antibodies to the deoxyribotrinucleotides dpApTpA and dpApApT were prepared by injecting the bovine serum albumin conjugates of the respective haptens in rabbits. The specificities of the antibodies were determined by estimating the inhibition of the binding of the tritiated haptens to the immunoglobulins by various nonradioactive mono- and oligonucleotides, using nitrocellulose membrane binding assay. Anti-dpApTpA and anti-dpApApT antisera were found to contain antibodies which were highly specific to the respective hapten sequence.
摘要
通过将相应半抗原的牛血清白蛋白缀合物注射到兔子体内，制备了针对脱氧核糖三核苷酸dpApTpA和dpApApT的抗体。使用硝酸纤维素膜结合测定法，通过估计各种非放射性单核苷酸和寡核苷酸对氚标记半抗原与免疫球蛋白结合的抑制作用，来确定抗体的特异性。发现抗dpApTpA和抗dpApApT抗血清含有对各自半抗原序列具有高度特异性的抗体。
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Antibodies to the codons ApApAp, ApApC, and ApUpG.针对密码子ApApAp、ApApC和ApUpG的抗体。
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Specific antibodies to dinucleotides and trinucleotides.针对二核苷酸和三核苷酸的特异性抗体。
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