McConnell D J
Nucleic Acids Res. 1979 Feb;6(2):525-44. doi: 10.1093/nar/6.2.525.
Restriction fragments of T7 DNA which selectively bind E. coli RNA polymerase have been identified. These include fragments located close to the beginning of gene 1 where according to Minkley and Pribnow (1973) there is a promoter called C. The smallest fragment from this region which binds RNA polymerase has been sequenced. It contains a promoter-like sequence, at an appropriate distance from the sequence TACA which Minkley and Pribnow suggested should lie at the initiation site of C. RNA synthesised in vitro from these fragments has been sequenced. The RNA sequence corresponds to the sequence to the right of the C promoter. The C promoter differs significantly from the A1 A2 and A3 promoters in sequence. Its structure and position suggest it plays a role in T7 infection.
已鉴定出可选择性结合大肠杆菌RNA聚合酶的T7 DNA限制性片段。其中包括靠近基因1起始处的片段，根据明克利和普里布诺（1973年）的研究，该区域有一个名为C的启动子。已对该区域结合RNA聚合酶的最小片段进行了测序。它包含一个类似启动子的序列，与明克利和普里布诺认为应位于C启动子起始位点的TACA序列相距适当距离。已对从这些片段体外合成的RNA进行了测序。RNA序列与C启动子右侧的序列相对应。C启动子在序列上与A1、A2和A3启动子有显著差异。其结构和位置表明它在T7感染中发挥作用。
