Siebenlist U
Nucleic Acids Res. 1979;6(5):1895-907. doi: 10.1093/nar/6.5.1895.
I have determined the nucleotide sequences of the three major early promoters of bacteriophage T7 (A1, A2, A3). The sequences confirm the two main homologies found between other known promoters for E. coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyl transferase, E.C. 2. 7. 7. 6). In particular, all three T7 promoters show a very good match with the -35 region homology; the A2 and A3 promoters share a 17 basepair sequence in this region. On the other hand, the match with the Pribnow Box homology is much less pronounced and different for each T7 promoter.
我已确定了噬菌体T7的三个主要早期启动子（A1、A2、A3）的核苷酸序列。这些序列证实了在大肠杆菌RNA聚合酶（核苷三磷酸：RNA核苷酸转移酶，E.C. 2. 7. 7. 6）的其他已知启动子之间发现的两个主要同源性。特别是，所有三个T7启动子与-35区域同源性都有很好的匹配；A2和A3启动子在该区域共享一个17碱基对的序列。另一方面，与Pribnow框同源性的匹配则不太明显，并且每个T7启动子都有所不同。
