Ren Jianjun, Xu Chuanbao, Shen Yunpeng, Li Chunyu, Dong Liping, Huhe Taoli, Zhi Junqiang, Wang Chongqing, Jiang Xingmei, Niu Dongze
Changzhou Key Laboratory of Biomass Green, Safe & High Value Utilization, Institute of Urban and Rural Mining, National-Local Joint Engineering Research Center for Biomass Refining and High-Quality Utilization, Changzhou University, Changzhou, 213164, China.
State Environmental Protection Engineering Center for Harmless Treatment and Resource Utilization of Antibiotic Fermentation Residues, Yili Chuanning Biotechnology Co., Ltd, Yili, 835007, China.
Environ Sci Pollut Res Int. 2023 May;30(24):65119-65128. doi: 10.1007/s11356-023-27087-x. Epub 2023 Apr 20.
With the growing concerns about antibiotic resistance, it is more and more important to prevent the environmental pollution caused by antibiotic fermentation residues. In this study, composted erythromycin fermentation residue (EFR) with the mixture of cattle manure and maize straw at ratios of 0:10 (CK), 1:10 (T1), and 3:10 (T2) explores the effects on physicochemical characteristics, mobile genetic elements (MGEs), and antibiotic resistance genes (ARGs). Results reflected that the addition of EFR reduced the carbon/nitrogen ratio of each compost and improved the piles' temperature, which promoted the composting process. However, the contents of Na, SO, and erythromycin were also significantly increased. After 30 days of composting, the degradation rates of erythromycin in CK, T1, and T2 were 72.7%, 20.3%, and 37.1%, respectively. Meanwhile, the total positive rates for 26 detected ARGs in T1 and T2 were 65.4%, whereas that of CK was only 23.1%. Further analysis revealed that ARGs responsible for ribosomal protection, such as ermF, ermT, and erm(35), dominated the composts of T1 and T2, and most were correlated with IS613, electrical conductivity (EC), nitrogen, and Zn. Above all, adding EFR helps to improve the nutritional value of composts, but the risks in soil salinization and ARG enrichment caused by high EC and erythromycin content should be further investigated and eliminated.
随着对抗生素耐药性的日益关注，防止抗生素发酵残渣造成的环境污染变得越来越重要。在本研究中，将红霉素发酵残渣（EFR）与牛粪和玉米秸秆按0:10（CK）、1:10（T1）和3:10（T2）的比例混合进行堆肥，探讨其对理化特性、可移动遗传元件（MGEs）和抗生素抗性基因（ARGs）的影响。结果表明，添加EFR降低了各堆肥的碳氮比，提高了堆体温度，促进了堆肥过程。然而，钠、硫和红霉素的含量也显著增加。堆肥30天后，CK、T1和T2中红霉素的降解率分别为72.7%、20.3%和37.1%。同时，T1和T2中26种检测到的ARGs的总阳性率为65.4%，而CK仅为23.1%。进一步分析表明，负责核糖体保护的ARGs，如ermF、ermT和erm(35)，在T1和T2的堆肥中占主导地位，且大多数与IS613、电导率（EC）、氮和锌相关。综上所述，添加EFR有助于提高堆肥的营养价值，但由高EC和红霉素含量引起的土壤盐渍化和ARG富集风险应进一步研究并消除。
