Tandon Ankita, Gulati Nikita, Singh Narendra Nath
Department of Oral Pathology, Microbiology and Forensic Odontology, Dental Institute, RIMS, Ranchi, Jharkhand, India.
Department of Oral Pathology and Microbiology, ITS-CDSR, Ghaziabad, Uttar Pradesh, India.
J Oral Maxillofac Pathol. 2022 Oct-Dec;26(4):589-590. doi: 10.4103/jomfp.jomfp_132_21. Epub 2022 Dec 22.
The oral mucous membrane is particularly sensitive to certain types of systemic disorders such as anemia, vitamin deficiencies, infectious diseases, hormonal disturbances and can be objectively reproduced through definite measurements using cytomorphometry.
The objective of the study is to evaluate the quantitative and qualitative changes in cytological buccal smears of obese individuals with type II diabetes (Group 1 = 20), obese individuals without type II diabetes (Group 2 = 20), individuals with type II diabetes without obesity (Group 3 = 20) by comparing with controls (individuals without obesity and without type II diabetes) (Group 4 = 20).
Buccal mucosal cells were scraped from study participants and were subjected to morphometric analysis (Magnus Pro software). Clinical history, hemoglobin A1c, heights and weights of participants were measured and consequently, their body mass index was calculated. Quantitative parameters (nuclear area, cytoplasmic area, nucleo-cytoplasmic ratio) and qualitative parameters (micronuclei [MN], nuclear budding, nuclear disintegration, apoptosis, necrosis) were assessed among the groups. The data were statistically interpreted using SPSS software version 20.0.
There is an increase in nuclear diameter and nuclear: cytoplasmic ratio of Groups 1 and 3 relative to Group 2. The qualitative assessment revealed MN and nuclear disintegration in Group 1 and 3 individuals. In addition, other qualitative changes such as nuclear budding and apoptotic bodies were evident in patients with type II diabetes.
The aforementioned qualitative and quantitative parameters facilitate early diagnosis and identification of individuals at risk of developing new age systemic illnesses such as diabetes and obesity.
口腔黏膜对某些全身性疾病特别敏感，如贫血、维生素缺乏、传染病、激素紊乱等，并且可以通过细胞形态计量学的明确测量客观地再现这些变化。
本研究的目的是通过与对照组（无肥胖和无II型糖尿病的个体）（第4组=20例）进行比较，评估II型糖尿病肥胖个体（第1组=20例）、无II型糖尿病肥胖个体（第2组=20例）、无肥胖的II型糖尿病个体（第3组=20例）口腔细胞学涂片的定量和定性变化。
从研究参与者中刮取颊黏膜细胞，并进行形态计量分析（Magnus Pro软件）。测量参与者的临床病史、糖化血红蛋白、身高和体重，并据此计算他们的体重指数。评估各组之间的定量参数（核面积、细胞质面积、核质比）和定性参数（微核[MN]、核出芽、核解体、凋亡、坏死）。使用SPSS 20.0软件对数据进行统计学解释。
相对于第2组，第1组和第3组的核直径和核质比增加。定性评估显示第1组和第3组个体存在微核和核解体。此外，II型糖尿病患者还存在其他定性变化，如核出芽和凋亡小体。
上述定性和定量参数有助于早期诊断和识别有患糖尿病和肥胖症等新时代全身性疾病风险的个体。
