Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea.
Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea.
Clin Chem Lab Med. 2023 Apr 24;61(10):1858-1866. doi: 10.1515/cclm-2023-0100. Print 2023 Sep 26.
The EasyCell assistant (Medica, Bedford, MA, USA) is one of the state-of-the-art digital morphology analyzers. We explored the performance of EasyCell assistant in comparison with manual microscopic review and Pentra DX Nexus (Horiba ABX Diagnostics, Montpellier, France).
In a total of 225 samples (100 normal and 125 abnormal samples), white blood cell (WBC) differentials and platelet (PLT) count estimation by EasyCell assistant were compared with the results by manual microscopic review and Pentra DX Nexus. The manual microscopic review was performed according to the Clinical and Laboratory Standards Institute guidelines (H20-A2).
WBC differentials between pre-classification by EasyCell assistant and manual counting showed moderate correlations for neutrophils (r=0.58), lymphocytes (r=0.69), and eosinophils (r=0.51) in all samples. After user verification, they showed mostly high to very high correlations for neutrophils (r=0.74), lymphocytes (r=0.78), eosinophils (r=0.88), and other cells (r=0.91). PLT count by EasyCell assistant highly correlated with that by Pentra DX Nexus (r=0.82).
The performance of EasyCell assistant for WBC differentials and PLT count seems to be acceptable even in abnormal samples with improvement after user verification. The EasyCell assistant, with its reliable performance on WBC differentials and PLT count, would help optimize the workflow of hematology laboratories with reduced workload of manual microscopic review.
EasyCell 助手（美国马萨诸塞州贝德福德的 Medica 公司）是最先进的数字形态分析仪之一。我们探索了 EasyCell 助手与手动显微镜检查和 Pentra DX Nexus（法国蒙彼利埃的 Horiba ABX Diagnostics）相比的性能。
在总共 225 个样本（100 个正常样本和 125 个异常样本）中，我们比较了 EasyCell 助手的白细胞（WBC）分类和血小板（PLT）计数与手动显微镜检查和 Pentra DX Nexus 的结果。手动显微镜检查根据临床和实验室标准协会指南（H20-A2）进行。
EasyCell 助手预分类与手动计数的 WBC 分类之间显示出中度相关性，所有样本中中性粒细胞（r=0.58）、淋巴细胞（r=0.69）和嗜酸性粒细胞（r=0.51）。在用户验证后，它们对于中性粒细胞（r=0.74）、淋巴细胞（r=0.78）、嗜酸性粒细胞（r=0.88）和其他细胞（r=0.91）显示出高度到非常高的相关性。EasyCell 助手的 PLT 计数与 Pentra DX Nexus 高度相关（r=0.82）。
EasyCell 助手在异常样本中的 WBC 分类和 PLT 计数性能似乎可以接受，在用户验证后得到改善。EasyCell 助手在 WBC 分类和 PLT 计数方面具有可靠的性能，将有助于优化血液学实验室的工作流程，减少手动显微镜检查的工作量。
