数字形态分析仪 Vision Pro 在白细胞分类中的性能。

Performance of digital morphology analyzer Vision Pro on white blood cell differentials.

机构信息

Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

Department of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Republic of Korea.

出版信息

Clin Chem Lab Med. 2021 Jan 20;59(6):1099-1106. doi: 10.1515/cclm-2020-1701. Print 2021 May 26.

DOI:10.1515/cclm-2020-1701

PMID:33470955

Abstract

OBJECTIVES

Vision Pro (West Medica, Perchtoldsdorf, Austria) is a recently developed digital morphology analyzer. We evaluated the performance of Vision Pro on white blood cell (WBC) differentials.

METHODS

In a total of 200 peripheral blood smear samples (100 normal and 100 abnormal samples), WBC preclassification and reclassification by Vision Pro were evaluated and compared with manual WBC count, according to the Clinical and Laboratory Standards Institute guidelines (H20-A2).

RESULTS

The overall sensitivity was high for normal WBCs and nRBCs (80.1-98.0%). The overall specificity and overall efficiency were high for all cell classes (98.1-100.0% and 97.7-99.9%, respectively). The absolute values of mean differences between Vision Pro and manual count ranged from 0.01 to 1.31. In leukopenic samples, those values ranged from 0.09 to 2.01. For normal WBCs, Vision Pro preclassification and manual count showed moderate or high correlations (r=0.52-0.88) except for basophils (r=0.34); after reclassification, the correlation between Vision Pro and manual count was improved (r=0.36-0.90).

CONCLUSIONS

This is the first study that evaluated the performance of Vision Pro on WBC differentials. Vision Pro showed reliable analytical performance on WBC differentials with improvement after reclassification. Vision Pro could help improve laboratory workflow.

摘要

目的

Vision Pro（奥地利威斯特梅迪卡公司，佩希托尔斯多夫）是一种新开发的数字形态分析仪。我们评估了 Vision Pro 在白细胞（WBC）分类中的性能。

方法

在总共 200 例外周血涂片样本（100 例正常和 100 例异常样本）中，根据临床和实验室标准协会指南（H20-A2），评估了 Vision Pro 对 WBC 预分类和重新分类的性能，并与手动 WBC 计数进行了比较。

结果

正常 WBC 和 nRBC 的总体敏感性较高（80.1-98.0%）。所有细胞类别的总体特异性和总体效率均较高（98.1-100.0%和 97.7-99.9%）。Vision Pro 与手动计数之间的平均差异绝对值范围为 0.01-1.31。在白细胞减少症样本中，这些值范围为 0.09-2.01。对于正常 WBC，Vision Pro 预分类和手动计数显示出中度或高度相关性（r=0.52-0.88），除嗜碱性粒细胞外（r=0.34）；重新分类后，Vision Pro 与手动计数之间的相关性得到改善（r=0.36-0.90）。