Oshimura M, Hesterberg T W, Barrett J C
Cancer Genet Cytogenet. 1986 Jul;22(3):225-37. doi: 10.1016/0165-4608(86)90159-7.
Cytogenetic studies were performed on eight early passage Syrian hamster embryo cell lines independently derived following asbestos exposure. The modal chromosome number of all the immortal cell lines was near-diploid. At the earliest passage examined, six of eight cell lines had only numerical chromosome changes. Cells in each of these six cell lines had an extra chromosome #11, either as a sole karyotypic change or with other numerical changes. The remaining two cell lines displayed both numerical and structural chromosome changes, but without involvement of chromosome #11. Common abnormalities were -X or -Y, +3, and 8p- in one cell line, and -13 and t(13;21) in the other cell line. A nonrandom gain of chromosome #8 was also found in four cell lines. In three of the four cell lines, trisomy of chromosome #8 seems to have occurred during karyotypic progression. The observation that nonrandom changes in chromosome number are an early karyotypic change after carcinogen treatment supports our hypothesis that induction of aneuploidy by asbestos is mechanistically important in the transformation of Syrian hamster embryo cells in culture and, further, suggests that trisomy 11 plays a major role in the early steps of immortalization and neoplastic progression.
对八个在接触石棉后独立衍生的早期传代叙利亚仓鼠胚胎细胞系进行了细胞遗传学研究。所有永生细胞系的众数染色体数接近二倍体。在检测的最早传代时，八个细胞系中的六个只有染色体数目变化。这六个细胞系中的每一个细胞系的细胞都有一条额外的11号染色体，要么是唯一的核型变化，要么伴有其他数目变化。其余两个细胞系显示出染色体数目和结构变化，但不涉及11号染色体。常见异常在一个细胞系中为-X或-Y、+3和8p-，在另一个细胞系中为-13和t(13;21)。在四个细胞系中还发现了8号染色体的非随机增加。在这四个细胞系中的三个中，8号染色体三体似乎在核型进展过程中发生。染色体数目非随机变化是致癌物处理后的早期核型变化这一观察结果支持了我们的假设，即石棉诱导非整倍体在培养的叙利亚仓鼠胚胎细胞转化中在机制上很重要，并且进一步表明11号染色体三体在永生化和肿瘤进展的早期步骤中起主要作用。
