Endo S, Metzler M, Hieber L
Strahlenbiologisches Institut, Ludwig-Maximilians-Universität München, Germany.
Carcinogenesis. 1994 Oct;15(10):2387-90. doi: 10.1093/carcin/15.10.2387.
Syrian hamster embryo (SHE) cells were used as a model system to study genetic changes during the rare spontaneous progression from normal to immortalized and then to neoplastically transformed cells. Cultures were established for immortalized and/or neoplastically transformed cells by inoculating 2-5 x 10(5) primary cells into 75 cm2 culture flasks and subsequent subculture at subconfluence. We examined the karyotypic changes in the spontaneously transformed and tumourigenic SHE cells. Chromosome analyses were performed on mitotic cells with the quinacrine banding technique. The primary SHE cell stock (82-6) was karyotypically normal, but cells that had overcome senescence exhibited chromosome abnormalities. More than 90% of cells from passages 18-85 carried the same deletion in the short arm of chromosome 2 (2p-). This deletion was also found in about 70% of cells analysed at passage 15. 2p- was never found in cells at passage 4. We further observed a variety of numeric and structural chromosome changes in addition to 2p-, but these were seen only in transformed cells at high passage numbers and in cell lines derived from nude mice tumours. Our findings suggest that 2p- predisposed SHE cells either to immortalization and/or to progression to tumourigenicity.
叙利亚仓鼠胚胎（SHE）细胞被用作模型系统，以研究从正常细胞罕见地自发进展为永生化细胞，然后再进展为肿瘤转化细胞过程中的遗传变化。通过将2 - 5×10⁵个原代细胞接种到75 cm²培养瓶中，并在亚汇合时进行后续传代培养，建立了永生化和/或肿瘤转化细胞的培养物。我们检查了自发转化的致瘤性SHE细胞的核型变化。采用喹吖因显带技术对有丝分裂细胞进行染色体分析。原代SHE细胞株（82 - 6）核型正常，但克服衰老的细胞表现出染色体异常。传代18 - 85的细胞中，超过90%在2号染色体短臂（2p-）上有相同的缺失。在传代15分析的细胞中，约70%也发现了这种缺失。在传代4的细胞中从未发现2p-。除了2p-，我们还进一步观察到各种染色体数目和结构的变化，但这些仅在高传代数的转化细胞以及源自裸鼠肿瘤的细胞系中可见。我们的研究结果表明，2p-使SHE细胞易于发生永生化和/或进展为致瘤性。
