Endo S, Hieber L
Strahlenbiologisches Institut, Ludwig-Maximilians-Universität München, Germany.
Int J Radiat Biol. 1995 Feb;67(2):177-86. doi: 10.1080/09553009514550221.
Primary Syrian hamster embryo (SHE) cells can be transformed in vitro by gamma-irradiation or spontaneously and display morphological alterations in discrete colonies as the earliest recognizable transformants. These morphologically transformed colonies can progress to give rise to immortal cell lines. The purpose of the present study was to determine if specific chromosome changes occur that correlate with immortalization. In the non-irradiated culture, 18 transformed colonies were isolated, of which two became immortal. In the irradiated culture, six out of 18 transformed colonies isolated progress to immortalization. Seven out of eight immortalized cell lines exhibited either numerical and/or structural chromosome alterations. Of six radiation-induced immortal lines, four lines showed rearrangements in the long arms of chromosome 3 (3q +) and chromosome 6 (6q +) non-randomly. In all cases, both 3q + and 6q + were detected in the primary transformed colonies from which the immortal cell lines arose. Both 3q + and 6q + occurred always as heterozygotes in the primary-transformed colonies. 3q + and 6q + were never found in the non-irradiated culture, demonstrating that these chromosome changes were induced by irradiation. 3q + and 6q + may correlate with progression to immortality in SHE cells transformed by gamma-irradiation.
原代叙利亚仓鼠胚胎（SHE）细胞可通过γ射线照射或自发地在体外发生转化，并在离散的集落中表现出形态改变，这是最早可识别的转化体。这些形态学上转化的集落可进一步发展形成永生细胞系。本研究的目的是确定是否发生了与永生相关的特定染色体变化。在未照射的培养物中，分离出18个转化集落，其中两个变成了永生细胞系。在照射的培养物中，分离出的18个转化集落中有6个发展为永生细胞系。8个永生细胞系中有7个表现出染色体数目和/或结构改变。在6个辐射诱导的永生细胞系中，有4个细胞系在3号染色体长臂（3q +）和6号染色体长臂（6q +）上出现了非随机重排。在所有情况下，永生细胞系起源的原代转化集落中均检测到3q +和6q +。在原代转化集落中，3q +和6q +总是以杂合子形式出现。在未照射的培养物中从未发现3q +和6q +，这表明这些染色体变化是由照射诱导的。3q +和6q +可能与γ射线照射转化的SHE细胞向永生的进展相关。
