Featured Laboratory for Biosynthesis and Target Discovery of Active Components of Traditional Chinese Medicine, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, 264003, China.
Yantai Key Laboratory of Pharmacology of Traditional Chinese Medicine in Tumor Metabolism, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai, 264003, China.
Microb Cell Fact. 2023 Apr 21;22(1):76. doi: 10.1186/s12934-023-02090-6.
Central carbon metabolism (CCM), including glycolysis, tricarboxylic acid cycle and the pentose phosphate pathway, is the most fundamental metabolic process in the activities of living organisms that maintains normal cellular growth. CCM has been widely used in microbial metabolic engineering in recent years due to its unique regulatory role in cellular metabolism. Using yeast and Escherichia coli as the representative organisms, we summarized the metabolic engineering strategies on the optimization of CCM in eukaryotic and prokaryotic microbial chassis, such as the introduction of heterologous CCM metabolic pathways and the optimization of key enzymes or regulatory factors, to lay the groundwork for the future use of CCM optimization in metabolic engineering. Furthermore, the bottlenecks in the application of CCM optimization in metabolic engineering and future application prospects are summarized.
中心碳代谢（CCM）包括糖酵解、三羧酸循环和磷酸戊糖途径，是维持生物体正常细胞生长的最基本代谢过程。由于其在细胞代谢中的独特调控作用，近年来已广泛应用于微生物代谢工程。以酵母和大肠杆菌为代表的生物体，我们总结了优化真核和原核微生物底盘中 CCM 的代谢工程策略，如引入异源 CCM 代谢途径和优化关键酶或调节因子，为未来 CCM 优化在代谢工程中的应用奠定基础。此外，还总结了 CCM 优化在代谢工程应用中的瓶颈和未来的应用前景。
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