Francis Anto Benignus, Settem Rajendra Prasad, Jeyamoorthy Moghanram, Nuthangi Venkata Harshith, Sharma Ashu, Rajasekharan Satish Kumar
Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu, India.
University at Buffalo, State University of New York, Buffalo, NY, United States.
Front Cell Infect Microbiol. 2025 Jun 27;15:1625103. doi: 10.3389/fcimb.2025.1625103. eCollection 2025.
This succinct article addresses the multifaceted interactions between the fungal organism and the Gram-positive bacterium in the development of oral biofilms and pathobiology of oral diseases. is considered to be a major pathogen in the development of dental caries. It is often found to interact with in oral infection settings. The interaction of these organisms is often mediated via the binding of Glucosyltransferase (GtfB) enzyme secreted by to surface proteins Als1 and Hwp1. During these interactions, both and exhibit increased gene regulatory activity, leading to the modulation of virulence attributes and adaptation to environmental changes. This results in the strong attachment of the species to tooth surfaces and increased resistance of the mixed species biofilms to external factors. Mechanistically, intercellular communication between these species in mixed biofilms through quorum sensing and production of exoenzymes such as glucosyltransferases account for the synergy and modulation of their virulence attributes. Specifically, these mixed-species biofilms exhibit increased acid production and enhanced resistance to antimicrobial agents. Understanding these complex interkingdom pattern of interactions is essential to develop efficient therapeutic approaches against biofilm-associated oral infections. The review also highlights probiotic strategies to interfere with these interkingdom interactions to combat oral diseases like early childhood caries (ECC).
这篇简洁的文章探讨了真菌生物体与革兰氏阳性细菌在口腔生物膜形成及口腔疾病病理生物学过程中的多方面相互作用。变形链球菌被认为是龋齿形成的主要病原体。在口腔感染环境中，常发现它与白色念珠菌相互作用。这些生物体之间的相互作用通常是通过变形链球菌分泌的葡糖基转移酶（GtfB）与白色念珠菌表面蛋白Als1和Hwp1的结合来介导的。在这些相互作用过程中，变形链球菌和白色念珠菌都表现出增强的基因调控活性，从而导致毒力特性的调节和对环境变化的适应。这导致这些菌种牢固附着于牙齿表面，并使混合菌种生物膜对外界因素的抵抗力增强。从机制上讲，混合生物膜中这些菌种之间通过群体感应和葡糖基转移酶等胞外酶的产生进行细胞间通讯，这解释了它们毒力特性的协同作用和调节。具体而言，这些混合菌种生物膜表现出产酸增加和对抗菌剂的抵抗力增强。了解这些复杂的跨界相互作用模式对于开发针对生物膜相关口腔感染的有效治疗方法至关重要。该综述还强调了益生菌策略，以干扰这些跨界相互作用来对抗诸如幼儿龋齿（ECC）等口腔疾病。
