Li Yanan, Chu Xue, Xie Xin, Guo Jinxiu, Meng Junjun, Si Qingying, Jiang Pei
Translational Pharmaceutical Laboratory, Jining First People's Hospital, Shandong First Medical University, Jining, China.
School of Pharmaceutical Sciences, Gannan Medical University, Ganzhou, China.
Front Mol Neurosci. 2023 Apr 6;16:1146525. doi: 10.3389/fnmol.2023.1146525. eCollection 2023.
Hypertension is a public health challenge worldwide due to its high prevalence and multiple complications. Hypertension-induced damage to the hippocampus leads to behavioral changes and various brain diseases. Despite the multifaceted effects of hypertension on the hippocampus, the mechanisms underlying hippocampal lesions are still unclear.
The 32-week-old spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats were selected as the study subjects. Behavioral experiments such as an open field test (OFT), an elevated plus maze (EPM) test, and the Morris water maze (MWM) test were performed to show the behavioral characteristics of the rats. A comprehensive transcriptomic and metabolomic analysis was performed to understand the changes in the hippocampus at the metabolic and genetic levels.
Behavioral tests showed that, compared to WKY rats, SHR showed not only reduced memory capacity but more hyperactive and impulsive behavior. In addition, transcriptomic analysis screened for 103 differentially expressed genes. Metabolomic analysis screened 56 metabolites with significant differences, including various amino acids and their related metabolites.
Comprehensive analysis showed that hypertension-induced hippocampal lesions are closely associated with differential metabolites and differential genes detected in this study. The results provide a basis for analyzing the mechanisms of hypertension-induced hippocampal damage.
高血压因其高患病率和多种并发症而成为全球公共卫生挑战。高血压对海马体造成的损伤会导致行为改变和各种脑部疾病。尽管高血压对海马体有多方面影响，但其导致海马体损伤的机制仍不清楚。
选取32周龄的自发性高血压大鼠（SHR）和Wistar-Kyoto（WKY）大鼠作为研究对象。进行旷场试验（OFT）、高架十字迷宫（EPM）试验和莫里斯水迷宫（MWM）试验等行为实验，以展示大鼠的行为特征。进行全面的转录组学和代谢组学分析，以了解海马体在代谢和基因水平上的变化。
行为测试表明，与WKY大鼠相比，SHR不仅记忆能力下降，而且行为更加多动和冲动。此外，转录组学分析筛选出103个差异表达基因。代谢组学分析筛选出56种有显著差异的代谢物，包括各种氨基酸及其相关代谢物。
综合分析表明，高血压诱导的海马体损伤与本研究中检测到的差异代谢物和差异基因密切相关。这些结果为分析高血压诱导海马体损伤的机制提供了依据。
