• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用 [F]-DPA-714 对暴露于蓝光的小鼠进行视网膜炎症的 PET 成像。

PET imaging of retinal inflammation in mice exposed to blue light using [F]-DPA-714.

机构信息

Department of Radiopharmaceuticals, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu Province, China.

NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine. Wuxi, Jiangsu Province, China.

出版信息

Mol Vis. 2022 Dec 31;28:507-515. eCollection 2022.

PMID:37089700
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10115360/
Abstract

PURPOSE

Positron emission tomography (PET) is widely used in high-precision imaging, which may provide a simple and noninvasive method for the detection of pathology and therapeutic effects. [F]-DPA-714 is a second-generation translocator protein (TSPO) positron emission tomography radiotracer that shows great promise in a model of neuroinflammation. In this study, [F]-DPA-714 micro-PET imaging was used to evaluate retinal inflammation in mice exposed to blue light, a well-established model of age-related macular degeneration (AMD) for molecular mechanism research and drug screening.

METHODS

C57BL/6J melanized mice were subjected to 10,000, 15,000, and 20,000 lux blue light for 5 days (8 h/day) to develop the retinal injury model, and the structure and function of the retina were assessed using hematoxylin-eosin (HE) staining, electroretinography (ERG), and terminal-deoxynucleotidyl transferase (TdT)-mediated nick-end labeling (TUNEL) immunostaining. Then, [F]-DPA-714 was injected approximately 100 μCi through each tail vein, and static imaging was performed 1 h after injection. Finally, the mice eyeballs were collected for biodistribution and immune analysis.

RESULTS

The blue light exposure significantly destroyed the structure and function of the retina, and the uptake of [F]-DPA-714 in the retinas of the mice exposed to blue light were the most significantly upregulated, which was consistent with the biodistribution data. In addition, the immunohistochemical, western blot, and immunofluorescence data showed an increase in microglial TSPO expression.

CONCLUSIONS

[F]-DPA-714 micro-PET imaging might be a good method for evaluating early inflammatory status during retinal pathology.

摘要

目的

正电子发射断层扫描（PET）在高精度成像中得到广泛应用，它可能为病理学和治疗效果的检测提供一种简单、无创的方法。[F]-DPA-714 是一种第二代转位蛋白（TSPO）正电子发射断层扫描放射性示踪剂，在神经炎症模型中显示出巨大的应用前景。在这项研究中，[F]-DPA-714 微 PET 成像用于评估暴露于蓝光的小鼠的视网膜炎症，这是一种成熟的年龄相关性黄斑变性（AMD）模型，用于分子机制研究和药物筛选。

方法

C57BL/6J 黑化小鼠暴露于 10000、15000 和 20000 勒克斯蓝光下 5 天（每天 8 小时），以建立视网膜损伤模型，使用苏木精-伊红（HE）染色、视网膜电图（ERG）和末端脱氧核苷酸转移酶（TdT）介导的缺口末端标记（TUNEL）免疫染色评估视网膜的结构和功能。然后，通过每条尾巴静脉注射约 100μCi [F]-DPA-714，注射后 1 小时进行静态成像。最后，收集小鼠眼球进行生物分布和免疫分析。

结果

蓝光照射显著破坏了视网膜的结构和功能，并且暴露于蓝光的小鼠视网膜中[F]-DPA-714 的摄取显著上调，这与生物分布数据一致。此外，免疫组织化学、western blot 和免疫荧光数据显示小胶质细胞 TSPO 表达增加。

结论

[F]-DPA-714 微 PET 成像可能是评估视网膜病变过程中早期炎症状态的良好方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/fa07334b953b/mv-v28-507-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/3be27b543089/mv-v28-507-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/6b47513f94a9/mv-v28-507-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/ce0fdd58b8d0/mv-v28-507-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/ebb4c9ce749f/mv-v28-507-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/fa07334b953b/mv-v28-507-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/3be27b543089/mv-v28-507-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/6b47513f94a9/mv-v28-507-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/ce0fdd58b8d0/mv-v28-507-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/ebb4c9ce749f/mv-v28-507-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a27/10115360/fa07334b953b/mv-v28-507-f5.jpg

相似文献

1
PET imaging of retinal inflammation in mice exposed to blue light using [F]-DPA-714.使用 [F]-DPA-714 对暴露于蓝光的小鼠进行视网膜炎症的 PET 成像。
Mol Vis. 2022 Dec 31;28:507-515. eCollection 2022.
2
PET imaging of retinal inflammation in mice exposed to blue light using [F]-DPA-714.使用 [F]-DPA-714 对暴露于蓝光下的小鼠进行视网膜炎症的 PET 成像。
Mol Vis. 2023 Jul 16;29:117-124. eCollection 2023.
3
C-DPA-713 Versus F-GE-180: A Preclinical Comparison of Translocator Protein 18 kDa PET Tracers to Visualize Acute and Chronic Neuroinflammation in a Mouse Model of Ischemic Stroke.C-DPA-713 与 F-GE-180：一种用于可视化缺血性中风小鼠模型中急性和慢性神经炎症的转位蛋白 18 kDa PET 示踪剂的临床前比较。
J Nucl Med. 2019 Jan;60(1):122-128. doi: 10.2967/jnumed.118.209155. Epub 2018 Jul 5.
4
Visualization of translocator protein (18 kDa) (TSPO) in the retina of diabetic retinopathy rats using fluorine-18-DPA-714.采用氟-18-DPA-714 对糖尿病视网膜病变大鼠视网膜中转位蛋白（18 kDa）（TSPO）进行可视化。
Ann Nucl Med. 2020 Sep;34(9):675-681. doi: 10.1007/s12149-020-01495-2. Epub 2020 Jul 6.
5
Combined [(18)F]DPA-714 micro-positron emission tomography and autoradiography imaging of microglia activation after closed head injury in mice.联合[(18)F]DPA - 714微正电子发射断层扫描及小鼠闭合性颅脑损伤后小胶质细胞激活的放射自显影成像
J Neuroinflammation. 2016 Jun 7;13(1):140. doi: 10.1186/s12974-016-0604-9.
6
[F]DPA-714 PET Imaging Reveals Global Neuroinflammation in Zika Virus-Infected Mice.[F]DPA-714 PET 成像显示寨卡病毒感染小鼠的全脑神经炎症。
Mol Imaging Biol. 2018 Apr;20(2):275-283. doi: 10.1007/s11307-017-1118-2.
7
Longitudinal PET imaging of muscular inflammation using 18F-DPA-714 and 18F-Alfatide II and differentiation with tumors.使用 18F-DPA-714 和 18F-Alfatide II 进行肌肉炎症的纵向 PET 成像及与肿瘤的鉴别。
Theranostics. 2014 Feb 26;4(5):546-55. doi: 10.7150/thno.8159. eCollection 2014.
8
In vivo imaging and characterization of [(18)F]DPA-714, a potential new TSPO ligand, in mouse brain and peripheral tissues using small-animal PET.使用小动物正电子发射断层扫描（PET）对潜在的新型转运蛋白18（TSPO）配体[(18)F]DPA - 714在小鼠脑和外周组织中的体内成像及特性研究
Nucl Med Biol. 2015 Mar;42(3):309-16. doi: 10.1016/j.nucmedbio.2014.11.009. Epub 2014 Dec 6.
9
Imaging microglial/macrophage activation in spinal cords of experimental autoimmune encephalomyelitis rats by positron emission tomography using the mitochondrial 18 kDa translocator protein radioligand [¹⁸F]DPA-714.通过正电子发射断层扫描术使用线粒体 18 kDa 转位蛋白放射性配体 [¹⁸F]DPA-714 对实验性自身免疫性脑脊髓炎大鼠脊髓中的小胶质细胞/巨噬细胞激活进行成像。
J Neurosci. 2012 Apr 25;32(17):5728-36. doi: 10.1523/JNEUROSCI.2900-11.2012.
10
PET imaging of neuroinflammation in a rat traumatic brain injury model with radiolabeled TSPO ligand DPA-714.放射性标记 TSPO 配体 DPA-714 对大鼠创伤性脑损伤模型神经炎症的 PET 成像。
Eur J Nucl Med Mol Imaging. 2014 Jul;41(7):1440-9. doi: 10.1007/s00259-014-2727-5. Epub 2014 Mar 11.

引用本文的文献

1
Impact of short wavelength light exposure on body weight, mobility, anxiety like behaviour and cytokine expression.短波长光照射对体重、活动能力、焦虑样行为和细胞因子表达的影响。
Sci Rep. 2025 Feb 18;15(1):5927. doi: 10.1038/s41598-025-89081-2.

本文引用的文献

1
LXA4 protects against blue-light induced retinal degeneration in human A2E-laden RPE cells and Balb-c mice.脂氧素A4可保护载有A2E的人视网膜色素上皮细胞和Balb-c小鼠免受蓝光诱导的视网膜变性。
Ann Transl Med. 2021 Aug;9(15):1249. doi: 10.21037/atm-21-3390.
2
Effect of a TSPO ligand on retinal pigment epithelial cholesterol homeostasis in high-fat fed mice, implication for age-related macular degeneration.高脂喂养小鼠 TSPO 配体对视网膜色素上皮胆固醇稳态的影响及其与年龄相关性黄斑变性的关系。
Exp Eye Res. 2021 Jul;208:108625. doi: 10.1016/j.exer.2021.108625. Epub 2021 May 20.
3
Applicability, potential and limitations of TSPO PET imaging as a clinical immunopsychiatry biomarker.
TSPO PET 成像作为临床免疫精神病学生物标志物的适用性、潜力和局限性。
Eur J Nucl Med Mol Imaging. 2021 Dec;49(1):164-173. doi: 10.1007/s00259-021-05308-0. Epub 2021 Mar 18.
4
Past, present and future role of retinal imaging in neurodegenerative disease.视网膜成像在神经退行性疾病中的过去、现在和未来作用。
Prog Retin Eye Res. 2021 Jul;83:100938. doi: 10.1016/j.preteyeres.2020.100938. Epub 2021 Jan 15.
5
PET evaluation of light-induced modulation of microglial activation and GLP-1R expression in depressive rats.正电子发射断层扫描评价光诱导对抑郁大鼠小胶质细胞激活和 GLP-1R 表达的调制作用。
Transl Psychiatry. 2021 Jan 6;11(1):26. doi: 10.1038/s41398-020-01155-z.
6
An update into the medicinal chemistry of translocator protein (TSPO) ligands.TSPO 配体的药物化学研究进展。
Eur J Med Chem. 2021 Jan 1;209:112924. doi: 10.1016/j.ejmech.2020.112924. Epub 2020 Oct 14.
7
Dietary Patterns, Carbohydrates, and Age-Related Eye Diseases.饮食模式、碳水化合物与年龄相关性眼病。
Nutrients. 2020 Sep 18;12(9):2862. doi: 10.3390/nu12092862.
8
Biomarkers for Alzheimer's Disease Early Diagnosis.用于阿尔茨海默病早期诊断的生物标志物
J Pers Med. 2020 Sep 4;10(3):114. doi: 10.3390/jpm10030114.
9
Visualization of translocator protein (18 kDa) (TSPO) in the retina of diabetic retinopathy rats using fluorine-18-DPA-714.采用氟-18-DPA-714 对糖尿病视网膜病变大鼠视网膜中转位蛋白（18 kDa）（TSPO）进行可视化。
Ann Nucl Med. 2020 Sep;34(9):675-681. doi: 10.1007/s12149-020-01495-2. Epub 2020 Jul 6.
10
The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye.TSPO-NOX1 轴控制吞噬细胞触发的眼部病理性血管生成。
Nat Commun. 2020 Jun 1;11(1):2709. doi: 10.1038/s41467-020-16400-8.