No apparent role for the Wari insulator in transcriptional regulation of the endogenous gene of .

Chromatin insulators have been proposed to play an important role in chromosome organization and local regulatory interactions. In , one of these insulators is known as Wari. It is located immediately downstream of the 3' end of the transcription unit. Wari has been proposed to interact with the promoter region, thereby facilitating recycling of the RNA polymerase machinery. We have tested this model by deleting the Wari insulator at the endogenous locus and could not detect a significant effect on eye pigmentation.