Kristyanto Sylvia, Jung Jaejoon, Kim Jeong Min, Kim Keunpil, Lee Mi-Hwa, Hao Lujiang, Jeon Che Ok
Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Freshwater Bioresources Utilization Division, Nakdonggang National Institute of Biological Resources, Gyeongsangbuk-do 37242, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005620.
Two Gram-stain-negative, catalase- and oxidase-positive, and aerobic bacteria, strains MSW7 and MSW13, were isolated from seawater. Cells of strains MSW7 and MSW13 are motile and non-motile rods, respectively. Strain MSW7 optimally grew at 25 °C and pH 7.0 and in the presence of 3 % (w/v) NaCl, whereas strain MSW13 optimally grew at 25 °C and pH 6.0-7.0 and in the presence of 2 % NaCl. As the sole respiratory quinone and the major fatty acids and polar lipids, strain MSW7 contained ubiquinone-8, C, C 8, C 8 and summed feature 3 (C 7 and/or C 6), and phosphatidylethanolamine and phosphatidylglycerol, respectively, whereas strain MSW13 contained menaquinone-6, C 6, iso-C, anteiso-C, and iso-C 3-OH, and phosphatidylethanolamine, respectively. The DNA G+C contents of strains MSW7 and MSW13 were 37.3 and 29.9 %, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains MSW7 and MSW13 were most closely related to A3 and WP25 with 98.8 and 98.1 % sequence similarities, respectively. The average nucleotide identity and digital DNA-DNA hybridization values between strain MSW7 and A3 and between strain MSW13 and KACC 17473 were 73.6 and 22.6 % and 80.4 and 23.8 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic data, strains MSW7 and MSW13 represent novel species of the genera and , respectively, for which the names sp. nov. and sp. nov. are proposed, respectively. The type strains of sp. nov. and sp. nov. are MSW7 (=KACC 22339=JCM 35001) and MSW13 (=KACC 22341=JCM 35021), respectively.
从海水中分离出两株革兰氏阴性、过氧化氢酶和氧化酶阳性的需氧细菌,菌株MSW7和MSW13。MSW7和MSW13菌株的细胞分别为运动型和非运动型杆菌。MSW7菌株在25 °C、pH 7.0以及3%(w/v)NaCl存在的条件下生长最佳,而MSW13菌株在25 °C、pH 6.0 - 7.0以及2% NaCl存在的条件下生长最佳。作为唯一的呼吸醌、主要脂肪酸和极性脂质,MSW7菌株分别含有泛醌-8、C、C 8、C 8和总和特征3(C 7和/或C 6),以及磷脂酰乙醇胺和磷脂酰甘油,而MSW13菌株分别含有甲基萘醌-6、C 6、异-C、anteiso-C和异-C 3-OH,以及磷脂酰乙醇胺。MSW7和MSW13菌株的DNA G+C含量分别为37.3%和29.9%。基于16S rRNA基因序列的系统发育分析表明,MSW7和MSW13菌株分别与A3和WP25关系最为密切,序列相似性分别为98.8%和98.1%。MSW7菌株与A3之间以及MSW13菌株与KACC 17473之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为73.6%和22.6%以及80.4%和23.8%。基于表型、化学分类和系统发育数据,MSW7和MSW13菌株分别代表了两个属的新物种,分别提出新名称sp. nov.和sp. nov.。sp. nov.和sp. nov.的模式菌株分别为MSW7(=KACC 22339=JCM 35001)和MSW13(=KACC 22341=JCM 35021)。