分析转录组特征，并鉴定灭活 COVID-19 疫苗保护作用的潜在基因。
Analysing transcriptomic signatures and identifying potential genes for the protective effect of inactivated COVID-19 vaccines.
机构信息
Department of Clinical Laboratory, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang University, Hangzhou, China.
Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Zhejiang University, Hangzhou, China.
出版信息
PeerJ. 2023 Apr 19;11:e15155. doi: 10.7717/peerj.15155. eCollection 2023.
Inactivated vaccines are one of the most effective strategies for controlling the coronavirus disease 2019 (COVID-19) pandemic. However, the response genes for the protective effect of inactivated vaccines are still unclear. Herein, we analysed the neutralization antibody responses elicited by vaccine serum and carried out transcriptome sequencing of RNAs isolated from the PBMCs of 29 medical staff receiving two doses of the CoronaVac vaccine. The results showed that SARS-CoV-2 neutralization antibody titers varied considerably among individuals, and revealed that many innate immune pathways were activated after vaccination. Furthermore, the blue module revealed that , , , and may be correlated with the protective effect of the inactivated vaccine. Additionally, , , , , and were demonstrated as the hub genes having a significant association with vaccines. These findings provide a basis for understanding the molecular mechanism of the host immune response induced by inactivated vaccines.
灭活疫苗是控制 2019 年冠状病毒病（COVID-19）大流行的最有效策略之一。然而，对于灭活疫苗的保护作用的应答基因仍不清楚。在此，我们分析了疫苗血清引起的中和抗体反应，并对 29 名接受两剂科兴疫苗的医务人员的 PBMCs 中的 RNA 进行了转录组测序。结果表明，个体之间的 SARS-CoV-2 中和抗体滴度差异很大，并显示接种疫苗后许多先天免疫途径被激活。此外，蓝色模块表明 、 、 、 和 可能与灭活疫苗的保护作用相关。此外， 、 、 、 、 和 被证明是与疫苗有显著关联的枢纽基因。这些发现为理解灭活疫苗诱导的宿主免疫反应的分子机制提供了基础。
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